贝那普利和氨氯地平对自发性高血压大鼠十二指肠、脑组织胆囊收缩素表达的影响

发布时间：2018-06-09 01:01

本文选题：自发性高血压大鼠 + 胆囊收缩素　；参考：《中华高血压杂志》2017年06期

【摘要】：目的探讨贝那普利和氨氯地平对自发性高血压大鼠(SHR)胆囊收缩素(CCK)mRNA及蛋白表达的影响。方法 45只14周龄雄性SHR随机分为SHR组(n=15)、贝那普利组[盐酸贝那普利灌服0.90mg/(kg·d),n=15]、氨氯地平组[苯磺酸氨氯地平灌服0.45mg/(kg·d),n=15],另以Wistar-Kyoto(WKY)大鼠作为正常对照组(n=15),SHR组及WKY组每日灌服同体积的蒸馏水,干预8周,测定各组大鼠安静清醒状态下尾动脉压,应用酶联免疫吸附试验(ELISA)法检测血清中CCK的含量;采用逆转录聚合酶链反应(RT-PCR)检测十二指肠中CCK mRNA表达;采用Western blot法检测十二指肠和脑中CCK蛋白表达。结果干预8周后,与WKY组比较,SHR组血压升高[收缩压/舒张压(201.0±14.6)/(157.4±16.2)比(136.3±12.0)/(102.9±10.2)mm Hg],十二指肠中CCK mRNA和蛋白表达显著降低,脑中CCK蛋白表达显著降低(均P0.05),但血清CCK水平差异无统计学意义(P0.05);与SHR组比较,贝那普利组与氨氯地平组血压降低[(181.5±17.5)/(145.4±18.3)、(188.4±14.5)/(145.6±13.8)比(201.0±14.6)/(157.4±16.2)mm Hg,均P0.05],贝那普利组与氨氯地平组十二指肠中CCK mRNA和蛋白表达、脑中CCK蛋白表达显著升高(均P0.05)。结论 SHR十二指肠和脑组织中CCK调节失衡,贝那普利和氨氯地平可能通过调节CCK的表达而降压。
[Abstract]:Objective to investigate the effects of benazepril and amlodipine on the mRNA and protein expression of cholecystokinin (CCK) in spontaneously hypertensive rats (SHR). Methods Forty-five 14-week-old male 0.90mg/(kg rats were randomly divided into three groups: benazepril group [benazepril hydrochloride], amlodipine sulfonic acid group [0.45mg/(kg daloxiandipine], and Wistar Kyoto WKYY rats as normal control group and WKY group. Distilled water of the same volume, After 8 weeks of intervention, the caudal arterial pressure of rats in quiet and awake state was measured, the serum CCK level was detected by Elisa, and the expression of CCK mRNA in duodenum was detected by reverse transcriptase polymerase chain reaction (RT-PCR) and reverse transcriptase polymerase chain reaction (RT-PCR). The expression of CCK protein in duodenum and brain was detected by Western blot. Results after 8 weeks of intervention, the blood pressure in SHR group was higher than that in WKY group [systolic blood pressure / diastolic blood pressure was 201.0 卤14.6 卤16.2 vs 136.3 卤12.0% / 102.9 卤10.2)mm Hg], and the expression of CCK mRNA and protein in duodenum was significantly lower than that in WKY group. The expression of CCK protein in brain was significantly lower than that in SHR group (P 0.05), but there was no significant difference in serum CCK level between SHR group and SHR group. The blood pressure decreased in benazepril group and amlodipine group [181.5 卤17.5 卤145.4 卤185.4 卤18.3C / P 145.6 卤13.8] compared with 201.0 卤14.6 卤147.4 卤16.2)mm Hg, respectively. The expression of CCK mRNA and protein in duodenum of benazepril group and amlodipine group was significantly higher than that of benazepril group and amlodipine group, and the expression of CCK protein in brain was significantly higher than that in benazepril group and amlodipine group (P 0.05). Conclusion CCK in duodenum and brain of SHR is out of balance. Benazepril and amlodipine may decrease the blood pressure by regulating the expression of CCK.
【作者单位】：甘肃中医药大学;甘肃省中医方药挖掘与创新转化重点实验室;
【基金】：国家自然科学基金资助项目(81160477) 甘肃省自然科学研究基金计划(1107RJZA220) 甘肃省高等学校基本科研业务费资助项目(2010-5)
【分类号】：R544.1

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