趋化因子CX3C受体1基因rs3732378多态性与急性冠状动脉综合征的相关性

发布时间：2018-06-09 13:58

  本文选题：趋化因子CXC受体 + 趋化因子CXC配体　； 参考：《中国动脉硬化杂志》2017年12期

【摘要】：目的探讨趋化因子CX3C受体1(CX3CR1)基因rs3732378单核苷酸多态性与急性冠状动脉综合征(ACS)的相关性。方法连续收集中国北方汉族人群951例,其中男性520例,女性431例,年龄35~75岁。根据冠状动脉造影(CAG)结果分为2组:(1)病例组(n=512):ACS患者;(2)对照组(n=439):非冠心病患者。病例组根据CAG检查血管病变支数分为3个亚组。采用测序法测定CX3CR1基因rs3732378单核苷酸多态位点的基因型。用多因素Logistic回归分析CX3CR1基因rs3732378多态性与ACS发病风险的关系。应用酶联免疫吸附法检测血浆中趋化因子CX3C配体1(CX3CL1)表达水平。结果两组CX3CR1基因rs3732378的基因型及等位基因的分布频率无显著性差异(P0.05)。rs3732378多态位点与ACS发病风险的总体和分层分析结果表明,CX3CR1基因rs3732378多态位点的3种基因型TT、TC和CC均不能增加ACS的发病风险(P0.05)。亚组分析显示,rs3732378多态位点的基因型和等位基因与冠状动脉血管病变支数无相关性(χ2=0.135,P=0.998;χ2=0.026,P=0.987)。病例组和对照组血浆中CX3CL1表达水平在rs3732378三种基因型无差异(P0.05)。结论 CX3CR1基因rs3732378多态位点不是ACS的易感基因,rs3732378多态性没有增加中国北方汉族人群ACS的风险。
[Abstract]:Objective to investigate the association between rs3732378 single nucleotide polymorphism of chemokine CX3C receptor (CX3C receptor 1) CX3CR1 gene and acute coronary syndrome (ACS). Methods 951 cases of Han nationality in northern China were collected, including 520 males and 431 females aged 35 to 75 years. According to the results of coronary angiography (CAG), the patients were divided into two groups: the control group (n = 512) and the control group (n = 439). The patients were divided into three subgroups according to the number of branches of angiopathy examined by CAG. The genotypes of rs3732378 single nucleotide polymorphisms of CX3 CR1 gene were determined by sequencing. Multivariate logistic regression analysis was used to analyze the relationship between CX3CR1 gene rs3732378 polymorphism and risk of ACS. Enzyme linked immunosorbent assay (Elisa) was used to detect the expression of CX3C ligand 1 (CX3CL1) in plasma. Results there was no significant difference in genotype and allelic frequency of CX3CR1 gene rs3732378 between the two groups. The overall and stratified analysis of the polymorphic locus rs3732378 and the risk of CX3CR1 gene rs3732378 showed that neither TTTC nor CC of CX3CR1 gene rs3732378 polymorphism locus was found. It can increase the risk of ACS (P 0.05). Subgroup analysis showed that there was no correlation between genotype and allele of rs3732378 polymorphic locus and the number of coronary artery lesion branches (蠂 ~ 2 + 0.135), 蠂 ~ (2 +) = 0.026 (P = 0.987). There was no difference in CX3CL1 expression among the three genotypes of rs3732378 between the case group and the control group (P 0.05). Conclusion the polymorphism of rs3732378 polymorphism of CX3CR1 gene does not increase the risk of ACS in Han population in northern China.
【作者单位】： 锦州医科大学研究生学院;沈阳军区总医院心血管内科;
【基金】：辽宁省科学技术计划面上项目(2015020400)
【分类号】：R541.4
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本文编号：1999961