间歇运动上调CTRP3表达抑制心梗大鼠心肌细胞凋亡及其机制

发布时间：2018-06-10 20:24

本文选题：间歇运动 + 心肌梗死　；参考：《陕西师范大学学报(自然科学版)》2017年03期

【摘要】：探讨间歇运动对心肌梗死(myocardial infarction,MI)大鼠C1q-肿瘤坏死因子相关蛋白3(CTRP3)表征及心肌细胞凋亡的影响。3月龄雄性SD大鼠30只,随机选取8只为假手术对照组(sham,S),S组只开胸穿线不结扎;余下大鼠永久性结扎左冠状动脉前降支,术后存活16只,随机分为心梗安静组(MI)和心梗+间歇运动组(ME),每组8只。ME组大鼠于术后1周进行为期8周的间歇运动训练,训练结束次日,腹腔麻醉取材。RT-qPCR检测CTRP3mRNA水平,Western Blot检测心肌CTRP3、p-AKT、AKT、p-mTOR、mTOR、细胞色素C、Bcl-2、Bax、BNP蛋白表达,TUNEL法检测心肌细胞凋亡,Masson染色分析心肌胶原容积(collagen volume fraction,CVF)百分比,血流动力学方法评定心功能。研究发现:心梗后大鼠心功能显著降低,心肌纤维化水平显著增加,CTRP3mRNA表达显著升高,蛋白表达显著降低,AKT和mTOR蛋白磷酸化上调,细胞色素C表达显著升高,Bcl-2/Bax比值降低,TUNEL阳性颗粒增加,BNP蛋白表达上调。间歇运动可显著改善心梗大鼠心功能,降低心肌纤维化水平,CTRP3基因与蛋白表达都显著升高,进一步上调AKT和mTOR蛋白磷酸化,降低细胞色素C蛋白表达,升高Bcl-2/Bax比值,减少TUNEL阳性颗粒,下调BNP的蛋白表达。间歇运动可升高心梗大鼠心肌中CTRP3水平,激活AKT/mTOR通路,抑制心肌细胞凋亡,改善心梗心脏病理性重塑,提高心功能。
[Abstract]:Effects of intermittent exercise on the characterization of C1q-TNF- related protein 3CTRP3) and the effect of intermittent exercise on cardiomyocyte apoptosis in rats with myocardial infarction, 30 male Sprague-Dawley (SD) rats aged 3 months were randomly selected as the sham operation control group (n = 8). Left anterior descending coronary artery was permanently ligated and 16 rats survived after operation. They were randomly divided into silent myocardial infarction group and intermittent myocardial infarction exercise group. Eight rats in each group received intermittent exercise training for 8 weeks one week after operation. The next day after training, CTRP3 mRNA level was detected by RT-PCR and CTRP3 mRNA level was detected by Western blot. The expression of cytochrome Bcl-2Bax-BNP protein was detected by Tunel method. Myocardial collagen volume and volume fractionation CVFpercentage were detected by Masson staining, and the cardiac function was evaluated by hemodynamic method. The results showed that after myocardial infarction, cardiac function was significantly decreased, myocardial fibrosis level was significantly increased, CTRP3 mRNA expression was significantly increased, and protein expression was significantly decreased in AKT and mTOR protein phosphorylation. The expression of cytochrome C increased significantly and the ratio of Bcl-2 / Bax decreased. Intermittent exercise significantly improved cardiac function, decreased the level of myocardial fibrosis and increased the expression of CTRP3 gene and protein, further up-regulated the phosphorylation of AKT and mTOR protein, decreased the expression of cytochrome C protein, and increased the ratio of Bcl-2 / Bax. Decreased Tunel positive granules and down-regulated BNP protein expression. Intermittent exercise could increase the level of CTRP3, activate AKT / mTOR pathway, inhibit myocardial cell apoptosis, improve the rational remodeling of myocardial infarction and improve cardiac function in myocardial infarction rats.
【作者单位】：陕西师范大学体育学院;
【基金】：国家自然科学基金(31371199,31171141)
【分类号】：R542.22

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