小鼠主动脉缩窄术后心肌水肿与水通道蛋白表达的相关性

发布时间：2018-06-19 21:42

本文选题：心肌肥厚 + 心肌水肿　；参考：《中国老年学杂志》2017年19期

【摘要】：目的观察小鼠心肌组织水通道蛋白(AQP)1的表达对心肌水肿的影响。方法采用主动脉缩窄法(TAC)建立小鼠心肌肥厚和水肿模型。实验分为四组:(1)假手术组(Sham组);(2)Sham+氯化汞(HgCl_2)组,假手术后心肌注射0.3 mmol/L HgCl_2 50μl;(3)手术组(TAC组);(4)TAC+HgCl_2组,TAC术后心肌注射0.3 mmol/L HgCl_2 50μl。每组10只。术后2 w心脏超声检测各组小鼠心功能。计算各组小鼠心脏重量/体重比和心肌组织含水量。采用RT-PCR法检测各组小鼠心肌组织中AQP1 mRNA表达。采用Western印迹法检测各组小鼠心肌组织AQP1蛋白表达。结果 TAC术后2 w,小鼠心肌组织发生明显肥厚和水肿。同时,TAC小鼠心肌组织中AQP1 mRNA和蛋白的表达比Sham组显著升高。心肌注射AQP1功能阻滞剂HgCl_2可以降低TAC小鼠心脏重量/体重比和心肌组织含水量,改善TAC小鼠心功能。结论心肌组织中AQP1表达增加与TAC术后心肌水肿密切相关,阻滞AQP1功能可减轻心肌水肿,并改善心功能。
[Abstract]:Objective to observe the effect of aquaporin aquaporin-1 on myocardial edema in mice. Methods the model of myocardial hypertrophy and edema in mice was established by coarctation of aorta. The experiment was divided into four groups: sham group (Sham group) and Sham group (HgCl2 group). After sham-operation, 0.3 mmol / L HgCl _ (250 渭 l / L) was injected into the myocardium after sham-operation) the TAC group was given 0.3 mmol / L HgCl _ (2) 50 渭 l 路L HgCl _ (2) after TAC. 10 rats in each group. Cardiac function was detected by echocardiography 2 weeks after operation. Heart weight / body weight ratio and myocardial water content were calculated. The expression of AQP1 mRNA in myocardium of each group was detected by RT-PCR. The expression of AQP1 protein in myocardium of each group was detected by Western blot. Results at 2 weeks after TAC, the myocardial tissue of the mice showed obvious hypertrophy and edema. At the same time, the expression of AQP1 mRNA and protein in myocardial tissue of TAC mice was significantly higher than that of Sham group. Myocardial injection of AQP1 functional blocker HgCl2 could reduce heart weight / body weight ratio and myocardial water content in TAC mice and improve cardiac function in TAC mice. Conclusion the increase of AQP1 expression in myocardial tissue is closely related to myocardial edema after TAC. Blocking AQP1 function can alleviate myocardial edema and improve cardiac function.
【作者单位】：盐城卫生职业技术学院;
【基金】：盐城市卫生局科技计划项目(No.YK2012040)
【分类号】：R542.2

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