分别转染TBX18后乳鼠成纤维细胞对心肌细胞搏动频率的影响

发布时间：2018-06-20 10:54

本文选题：TBX + 成纤维细胞　；参考：《华中科技大学学报(医学版)》2017年01期

【摘要】：目的用TBX18分别转染成纤维细胞(CFs)与心肌细胞(NRVMs),探究转染的成纤维细胞对整个共培养系统的影响。方法用TBX18分别转染乳鼠心脏CFs与NRVMs,并分成5组:NRVMs组,TBX18-NRVMs组,TBX18-NRVMs+CFs组,TBX18-NRVMs+GFP(绿色荧光蛋白)-CFs组与TBX18-NRVMs+TBX18-CFs组。培养7d后观察并比较各组细胞的搏动频率。结果共培养7d后发现TBX18可显著提高心肌细胞的自发搏动[(94.20±7.38)b/min vs.(62.10±11.67)b/min,P0.01],且相比TBX18-NRVMs组,GFP-CFs或CFs与TBX18-NRVMs共培养并没有明显改变搏动频率,但CFs具有减缓心肌自发搏动的趋势,经TBX18转染后的CFs与TBX18-NRVMs共培养可显著提高心肌细胞的搏动频率[(118.50±7.25)b/min vs.(82.80±15.82)b/min,P0.01]。结论在分别转染TBX18的成纤维细胞与心肌细胞共培养系统中,成纤维细胞可显著提高心肌细胞的搏动频率。
[Abstract]:Objective to investigate the effect of TBX18 on the whole co-culture system by transfection of fibroblasts (CFS) and cardiomyocytes (NRVMs1) respectively. Methods CFS and NRVMswere transfected with TBX18, respectively, and divided into 5 groups: TBX18-NRVMs (TBX18-NRVMs CFS) and TBX18-NRVMs (TBX18-NRVMs GFPs) and TBX18-NRVMs TBX18-CFS (TBX18-NRVMs). After 7 days of culture, the pulsatile frequency of each group was observed and compared. Results after 7 days of co-culture, it was found that TBX18 could significantly increase the spontaneous pulsation of cardiomyocytes [94.20 卤11.67 7.38)b/min vs.(62.10 卤11.67 b / min P0.01]. Compared with TBX18-NRVMs, TBX18-NRVMs co-cultured with GFP-CFs or CFS did not significantly change the pulsatile frequency, but CFS had a tendency to slow down myocardial spontaneous pulsation. The co-culture of CFS and TBX18-NRVMs transfected with TBX18 significantly increased the pulsatile frequency of cardiomyocytes [118.50 卤7.25)b/min vs.(82.80 卤15.82mb / min P0.01]. Conclusion in the co-culture system of fibroblasts transfected with TBX18, fibroblasts can significantly increase the pulsatile frequency of cardiomyocytes.
【作者单位】：武汉大学人民医院心内科武汉大学心血管研究所湖北省心血管重点实验室;湖北省十堰市太和医院心内科;
【基金】：国家自然科学基金资助项目(No.81070142) 湖北省科技支撑计划资助项目(No.2013BCB013)
【分类号】：R54

【相似文献】