过表达缺氧诱导因子1α心肌干细胞能提高心肌梗死后心功能及移植细胞的存活率

发布时间：2018-06-27 01:16

本文选题：心肌梗塞 + 缺氧诱导因子　；参考：《中国组织工程研究》2017年01期

【摘要】：背景:干细胞移植后最大的障碍是细胞在缺血、缺氧等恶劣环境下极低的存活率。为解决这一难题,人们尝试基因修饰的方法,即在细胞移植前将抗凋亡基因通过转染或转导的方式,使其基因结构发生改变,进而可能会从根源上解决干细胞的存活、代谢及增生或分化等能力。目的:经缺氧诱导因子1α基因修饰的心肌干细胞移植入大鼠心肌梗死边缘区后,观察移植后心肌干细胞的存活率及大鼠心功能的变化。方法:将24只SD大鼠随机等分为3组:缺氧诱导因子1α转染心肌干细胞组、心肌干细胞组和模型组,以开胸结扎冠脉的方法制备心肌梗死模型,2周后分别注射经Ad-HIF1α-eGFP转染的心肌干细胞、Ad-eGFP转染的心肌干细胞及PBS液。结果与结论:心肌干细胞移植可提高心功能;经缺氧诱导因子1α基因修饰的心肌干细胞移植可较单纯心肌干细胞移植更显著改善心功能;梗死后左室射血分数的改善与梗死边缘区心肌干细胞密度呈正相关。说明过表达缺氧诱导因子1α的心肌干细胞能有效改善心肌梗死大鼠心功能及移植细胞的存活。
[Abstract]:Background: the biggest obstacle after stem cell transplantation is the very low survival rate of cells in poor conditions such as ischemia and hypoxia. In order to solve this problem, people try to modify the gene, that is, to change the gene structure of anti-apoptotic gene by transfection or transduction before cell transplantation, which may solve the root cause of stem cell survival. Metabolism and ability to proliferate or differentiate. Aim: to observe the survival rate and cardiac function of myocardial stem cells modified by hypoxia inducible factor 1 伪 gene after transplantation into the marginal area of myocardial infarction in rats. Methods: Twenty-four SD rats were randomly divided into three groups: hypoxia inducible factor-1 伪 transfected myocardial stem cells group, myocardial stem cell group and model group. Myocardial infarction model was established by opening and ligating the coronary artery. After 2 weeks, the myocardial stem cells transfected with Ad-HIF1 伪 -eGFP and PBS solution were injected with Ad-eGFP respectively. Results and conclusion: myocardial stem cell transplantation can improve cardiac function, and hypoxia inducible factor 1 伪 gene modified myocardial stem cell transplantation can improve cardiac function more significantly than myocardial stem cell transplantation alone. The improvement of left ventricular ejection fraction was positively correlated with myocardial stem cell density. These results suggest that myocardial stem cells overexpression of hypoxia inducible factor-1 伪 can effectively improve cardiac function and survival of transplanted cells in rats with myocardial infarction.
【作者单位】：河北医科大学附属河北省人民医院心内一科;
【分类号】：R394.2

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