Galectin-3在缺血性心功能不全家兔心室重构中的作用

发布时间：2018-06-28 18:36

本文选题：半乳糖凝集素 + 心力衰竭　；参考：《中国病理生理杂志》2017年07期

【摘要】：目的:探讨半乳糖凝集素3(galectin-3,Gal-3)与心肌纤维化的关系,明确Gal-3在缺血性心功能不全家兔心室重构中的作用。方法:采用结扎冠状动脉前降支的方法制作缺血性心功能障碍家兔模型。利用随机数字表法将20只家兔随机分为假手术组和心功能不全组。造模成功4周后采用心脏超声测定心功能;real-time PCR和Western blot方法分别检测心肌中Gal-3、Ⅰ型胶原和Ⅲ型胶原的mRNA表达和蛋白含量;ELISA法检测家兔血清中Gal-3水平;采用HE染色和Masson染色法检测家兔梗死区心肌组织的纤维化程度。结果:与假手术组相比,心功能不全组梗死区心肌组织Gal-3的mRNA和蛋白表达明显增加,与此同时Ⅰ型胶原和Ⅲ型胶原的mRNA和蛋白表达也明显增加;血清Gal-3水平明显上升;病理学观察可见心功能不全组的心肌细胞形态不一、排列紊乱,纤维组织明显增生。结论:Gal-3加重了缺血性心功能不全家兔的心肌纤维化,促进了心室重构和心衰的发生。
[Abstract]:Objective: To investigate the relationship between galectin-3 (galectin-3, Gal-3) and myocardial fibrosis, and to clarify the role of Gal-3 in ventricular remodeling in ischemic cardiac insufficiency. Methods: a rabbit model of ischemic cardiac dysfunction was made by ligating the anterior descending branch of coronary artery. 20 rabbits were randomly divided into sham operation with random digital table method. The cardiac function was measured by echocardiography after 4 weeks of success. Real-time PCR and Western blot were used to detect Gal-3, mRNA expression and protein content of type I collagen and type III collagen, ELISA method was used to detect the level of Gal-3 in rabbit serum, and HE staining and Masson staining were used to detect the myocardial infarction group in rabbits. Results: compared with the sham group, the expression of mRNA and protein in the myocardial tissue Gal-3 in the infarcted area was obviously increased, while the mRNA and protein expression of type I collagen and type III collagen increased obviously, the level of serum Gal-3 increased obviously, and the pathological observation of cardiac insufficiency group showed the morphology of cardiac myocytes in the group of cardiac dysfunction. Conclusion: Gal-3 aggravates myocardial fibrosis in ischemic heart dysfunction rabbits and promotes the occurrence of ventricular remodeling and heart failure.
【作者单位】：河北医科大学附属河北省人民医院心内一科;
【分类号】：R541.6;R542.2

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