牛磺酸减轻内毒素诱导的大鼠心肌损伤

发布时间：2018-06-30 17:46

本文选题：牛磺酸 + 脂多糖　；参考：《中国病理生理杂志》2017年01期

【摘要】：目的:探讨牛磺酸对内毒素(即脂多糖,LPS)诱导的大鼠心肌损伤的影响。方法:健康雄性Sprague-Dawley(SD)大鼠30只随机分为3组:正常对照组、内毒素模型组及牛磺酸处理组。正常对照组和内毒素模型组大鼠尾静脉注射生理盐水,牛磺酸处理组大鼠尾静脉注射牛磺酸(100 mg/kg),2 h后,内毒素模型组和牛磺酸处理组大鼠腹腔注射LPS(10 mg/kg),正常对照组大鼠腹腔注射生理盐水。注射内毒素6 h后,采集血样品和心肌组织,检测血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、肿瘤坏死因子α(TNF-α)及白细胞介素6(IL-6)水平;光镜下观察心肌形态学变化;Western blot检测心肌组织磷酸化核因子κB(p-NF-κB)、环氧合酶2(COX-2)、TNF-α、IL-6及血红素加氧酶1(HO-1)的表达。结果:与正常对照组比较,内毒素模型组大鼠血清SOD活性及心肌组织HO-1表达明显降低(P0.01),血清MDA、TNF-α和IL-6水平明显升高(P0.01),心肌组织p-NF-κB、COX-2、TNF-α及IL-6水平明显升高(P0.01)。与内毒素模型组比较,牛磺酸处理组大鼠血清MDA、TNF-α和IL-6水平明显降低(P0.01),牛磺酸处理明显降低心肌组织COX-2、TNF-α、IL-6及p-NF-κB水平(P0.01),血清SOD活性及心肌组织HO-1表达明显提高(P0.01)。组织学观察显示内毒素模型组大鼠心肌组织有炎症细胞浸润,心肌纤维排列疏松不规则,而正常对照组和牛磺酸处理组大鼠心肌纤维排列整齐规则。结论:牛磺酸预处理能减轻内毒素诱导的心肌损伤,其机制可能通过HO-1/CO信号下调p-NF-κB/COX-2而发挥作用。
[Abstract]:Aim: to investigate the effect of taurine on myocardial injury induced by lipopolysaccharide (LPS) in rats. Methods: thirty healthy male Sprague-Dawley (SD) rats were randomly divided into three groups: normal control group, endotoxin model group and taurine treatment group. Rats in normal control group and endotoxin model group were injected with normal saline via tail vein, and taurine (100 mg/kg) was injected into tail vein of taurine treatment group for 2 h. Rats in endotoxin model group and taurine treatment group were intraperitoneally injected with LPS (10 mg/kg), and normal control rats were intraperitoneally injected with normal saline. Serum superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, tumor necrosis factor 伪 (TNF- 伪) and interleukin-6 (IL-6) levels were measured 6 h after endotoxin injection. The expression of phosphorylated nuclear factor- 魏 B (p-NF- 魏 B), cyclooxygenase-2 (COX-2), TNF- 伪, and heme oxygenase-1 (HO-1) in myocardium were detected by blot under light microscope. Results: compared with the normal control group, the serum SOD activity and HO-1 expression in the endotoxin group were significantly decreased (P0.01), the levels of serum MDA-TNF- 伪 and IL-6 were significantly increased (P0.01), and the levels of p-NF- 魏 BCK-COX-2TNF- 伪 and IL-6 were significantly increased (P0.01). Compared with the endotoxin model group, the levels of serum MDA-TNF- 伪 and IL-6 in taurine treated rats were significantly decreased (P0.01), the levels of IL-6 and p-NF- 魏 B in myocardial tissue were significantly decreased by taurine treatment (P0.01), the activity of serum SOD and the expression of HO-1 in myocardial tissue were significantly increased (P0.01). Histological observation showed that there were inflammatory cell infiltration and irregular arrangement of myocardial fibers in the endotoxin model group, while the arrangement of myocardial fibers in the normal control group and taurine treatment group was regular. Conclusion: taurine pretreatment can attenuate the myocardial injury induced by endotoxin, and its mechanism may be played by down-regulation of p-NF- 魏 B / COX-2 by HO-1 / CO signal.
【作者单位】：芜湖市第一人民医院心血管内一科;皖南医学院机能实验中心;皖南医学院病理生理教研室;皖南医学院生理教研室;
【基金】：国家自然科学基金资助项目(No.81172790)
【分类号】：R54

【参考文献】