NECA对大鼠离体缺血再灌注心肌细胞自噬的调控作用

发布时间：2018-07-03 17:31

本文选题：NECA + 对大　；参考：《山东医药》2017年22期

【摘要】：目的观察非选择性腺苷受体激动剂5'-N-乙基酰胺基腺苷(NECA)对离体心肌缺血再灌注大鼠细胞自噬的调控作用。方法将42只Wistar大鼠随机分为Sham组、I/R组、NECA组,各14只。Sham组心脏只穿线但不结扎缺血,用Kerbs-Henseleit缓冲液持续灌流170 min。I/R组、NECA组使用Langendorff装置制备大鼠离体心肌缺血再灌注损伤模型,且NECA组再灌注前分别给予含NECA的灌流液。透射电镜观察各组大鼠心肌超微结构的改变;Western blotting法检测自噬标志蛋白Beclin-1、LC3的表达。结果 Sham组、NECA组透射电镜下未见自噬体存在,但在I/R组可见多处典型的自噬现象。与Sham组相比,I/R组Beclin-1蛋白表达高、LC3Ⅱ/LC3Ⅰ高(P均0.05)。与I/R组相比,NECA组Beclin-1蛋白表达低、LC3Ⅱ/LC3Ⅰ低(P均0.05)。结论 NECA可通过降低大鼠心肌细胞自噬发挥心肌保护作用。
[Abstract]:Aim to investigate the effects of 5-N- ethyladenosine (NECA), a nonselective adenosine receptor agonist, on autophagy in isolated myocardial ischemia reperfusion rats. Methods Forty-two Wistar rats were randomly divided into the Sham group (n = 14) and the control group (n = 14). The hearts of each group were perfused continuously with Kerbs-Henseleit buffer solution for 170 min 路I / R group. The isolated myocardial ischemia-reperfusion injury model was established by Langendorff device in the NECA group. NECA group was given NECA solution before reperfusion. The changes of myocardial ultrastructure in each group were observed by transmission electron microscope. The expression of the autophagy marker protein Beclin-1 (LC3) was detected by Western blotting. Results No autophagy was observed under transmission electron microscope in Sham group, but many typical autophagy phenomena were observed in I / R group. The expression of Beclin-1 protein in I / R group was higher than that in Sham group (P 0.05). Compared with I / R group, Beclin-1 protein expression in NECA group was lower than that in I / R group (P 0.05). Conclusion NECA can protect myocardium by decreasing autophagy of rat cardiac myocytes.
【作者单位】：华北理工大学护理与康复学院;
【基金】：河北省医学科学研究重点课题计划(20090548)
【分类号】：R542.22

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