apelin干预骨髓间充质干细胞在缺血缺氧条件下的生存和血管再生

发布时间：2018-07-10 00:37

本文选题：骨髓 + 间质干细胞　；参考：《中国组织工程研究》2017年01期

【摘要】：背景:课题组前期研究发现骨髓间充质干细胞移植能够改善大鼠心肌梗死后心功能,但整体效果并不太理想,骨髓间充质干细胞在心肌梗死组织局部的生存力和新生血管密度低下。目的:观察血管紧张素受体AT1相关的受体蛋白内源性配体(apelin)对骨髓间充质干细胞在缺血缺氧条件下的生存和血管形成能力的影响并探讨相关机制。方法:体外培养的骨髓间充质干细胞分为骨髓间充质干细胞组和骨髓间充质干细胞+apelin组,分别于正常条件(完全培养基,体积分数为20%O_2)和缺血缺氧条件(无血清,体积分数为1%O_2)下培养24 h,其中骨髓间充质干细胞+apelin组在培养同时加入apelin-13(5μmol/L)刺激骨髓间充质干细胞。MTS法检测细胞增殖能力,TUNEL法检测细胞凋亡情况。取细胞培养液分别刺激人脐静脉内皮细胞,观察骨髓间充质干细胞血管形成能力。Western blot检测骨髓间充质干细胞中血管内皮生长因子的表达。结果与结论:与骨髓间充质干细胞组相比较,骨髓间充质干细胞+apelin组正常条件和缺血缺氧条件下扩增能力显著增强,凋亡减少,血管管腔样结构明显增多,血管内皮生长因子的表达明显增高。以上结果提示apelin能够增强骨髓间充质干细胞在缺血缺氧环境下的生存和血管形成能力,此效应可能与其上调血管内皮生长因子的表达相关。
[Abstract]:Background: our previous study found that bone marrow mesenchymal stem cell transplantation can improve cardiac function after myocardial infarction in rats, but the overall effect is not very satisfactory. Bone marrow mesenchymal stem cells (BMSCs) have low local viability and neovascularization in myocardial infarction. Aim: to investigate the effects of angiotensin receptor AT1-associated receptor protein endogenous ligand (apelin) on the survival and angiogenesis of bone marrow mesenchymal stem cells under ischemia and hypoxia. Methods: bone marrow mesenchymal stem cells cultured in vitro were divided into two groups: bone marrow mesenchymal stem cell group and bone marrow mesenchymal stem cell apelin group. Bone marrow mesenchymal stem cells (apelin group) were cultured for 24 hours under the volume fraction of 1 and 20 渭 mol / L respectively. The proliferation of bone marrow mesenchymal stem cells (BMSCs) was detected by apelin-13 (5 渭 mol / L). The vascular endothelial growth factor (VEGF) expression in bone marrow mesenchymal stem cells (BMSCs) was detected by Western blot. Results and conclusion: compared with the bone marrow mesenchymal stem cells (BMSCs) group, the expansion ability of BMSCs in apelin group was significantly enhanced, apoptosis was decreased, and vascular lumen structure was significantly increased in apelin group. The expression of vascular endothelial growth factor (VEGF) was significantly increased. These results suggest that apelin can enhance the survival and angiogenesis of bone marrow mesenchymal stem cells under ischemia and hypoxia, which may be related to the up-regulation of vascular endothelial growth factor expression.
【作者单位】：中山大学孙逸仙纪念医院急诊科;
【基金】：国家自然科学基金(81070125)“抗凋亡与促血管生成miRNA-378干预MSCs治疗心梗后心衰的机制研究”;国家自然科学基金(81270213)“ANGⅡ/AT1/SMAD/CX43通路在心肌干细胞提高心梗大鼠心电生理学稳定性和室颤阈值的作用机制研究”;国家自然科学基金(81670306)“心肌干细胞经IGF-1/STAT/miR-155通路下调AT1R改善心肌梗死后心电生理稳定性机理研究” 广东省科技计划项目(2014A020211002)“LncRNA-Bvht/MESP1/N-cadherin通路调控MSCs向心肌细胞定向分化的机制研究”;广东省科技计划项目(2010B031600032)“抗凋亡与促血管生成miRNA-378干预MSCs治疗心梗后心衰的机制研究” 高校基本科研业务费中山大学青年教师重点培育项目(13ykzd16)“PPAR-γ/TGF-β1/Smad/CX43通路在PPAR-γ干预MSCs治疗心梗后心衰的疗效及机制研究” 广东省医学科研基金(A2016264)“心肌干细胞经由HIF-1α/Apelin/APJ/ACE2通路下调ANGII改善心肌梗死大鼠心电生理学稳定性的机制研究”~~
【分类号】：R54

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