通心络对兔腹主动脉粥样硬化易损斑块中三维应变的影响

发布时间：2018-07-13 19:48

【摘要】：目的在兔腹主动脉粥样易损斑块硬化模型中,以辛伐他汀作为阳性对照,进一步明确通心络对易损斑块三维应变的影响。方法 50只纯种雄性新西兰大白兔行腹主动脉球囊拉伤,1%胆固醇饲料喂养10周,改为普通饲料继续喂养8周,并随机分为对照组、小剂量通心络组[0.15 g/(kg·d)]、中剂量通心络组[0.3 g/(kg·d)]、高剂量通心络组[0.6 g/(kg·d)]和辛伐他汀组[5 mg/(kg·d)],每组10只。18周末采用酶法检测其血脂水平,并行体表三维超声及血管内超声检查,处死动物,取腹主动脉脱水,包埋制石蜡及冰冻切片,行苏木精-伊红染色、组织病理学染色以及免疫组织化学染色。结果在通心络和辛伐他汀药物干预下,中、大剂量通心络组及辛伐他汀组动脉粥样硬化斑块的周向(CSp)和轴向应变峰值(RSp)降低,而纵向应变峰值(LSp)升高,斑块面积和斑块负荷减小。同时,中、大剂量通心络及辛伐他汀干预可以减少斑块内巨噬细胞含量,降低斑块易损指数,并且减少斑块内基质金属蛋白酶2、9(MMP-2,MMP-9)的表达。结论通心络和辛伐他汀可通过抑制斑块内巨噬细胞含量及MMP-2、MMP-9表达影响斑块的三维应变,稳定动脉粥样硬化斑块,其稳定斑块作用可通过检测斑块三维应变进行无创监测。
[Abstract]:Objective to determine the effect of simvastatin on the three-dimensional strain of vulnerable plaque in the rabbit abdominal aorta atherosclerotic plaque hardening model. Methods the effect of Tongxinluo on the three-dimensional strain of vulnerable plaques was further clarified. Methods 50 male New Zealand white rabbits were treated with abdominal aorta balloon injury and 1% cholesterol feed for 10 weeks. In the control group, the low dose Tongxinluo group [0.15 g/ (kg. D)], the middle dose Tongxinluo group [0.3 g/ (kg. D)], the high dose Tongxinluo group [0.6 g/ (kg d) and the simvastatin group, were used to detect the blood lipid level by enzyme method, and the animals were killed and the abdominal aorta was dehydrated, and the abdominal aorta was dehydrated, and the abdominal aorta was dehydrated. Embedded paraffin and frozen sections were stained with hematoxylin eosin, histopathology and immunohistochemical staining. Results in the intervention of Tongxinluo and simvastatin, the circumferential (CSp) and axial strain peak value (RSp) of atherosclerotic plaques in the large dose Tongxinluo group and the simvastatin group were reduced, while the peak strain peak (LSp) rose in the large dose of Tongxinluo group and simvastatin group. High dose of plaque area and plaque load decrease. Meanwhile, large dose of Tongxinluo and simvastatin can reduce the content of macrophage in plaque, reduce the vulnerability index of plaque, and reduce the expression of matrix metalloproteinase 2,9 (MMP-2, MMP-9) in plaque. Conclusion Tongxinluo and simvastatin can inhibit the content of macrophages and MMP in plaque. The expression of -2 and MMP-9 can affect the three-dimensional strain of plaque and stabilize atherosclerotic plaques. The stable plaque effect can be detected by non-invasive monitoring of three-dimensional strain of plaque.
【作者单位】：山东大学齐鲁医院心内科教育部与卫生部心血管功能与重构重点实验室;新泰市第二人民医院介入治疗室;济南市第四人民医院超声科;
【基金】：国家重点基础研究发展计划(973计划,2012CB518603) 国家自然科学基金(60971023,81270351)
【分类号】：R543.5

【相似文献】