利用流式细胞仪检测两性霉素B依赖性抗体

发布时间：2018-07-28 06:56

【摘要】：目的建立流式细胞术检测药物依赖性血小板抗体的标准方法。方法针对1名再生障碍性贫血患者的血小板抗体检测,通过ELISA法检测其血浆中和血小板上特异性和自身抗体,用Lifecodes LSATM Class I(Luminex试剂盒,磁珠液相悬浮芯片系统)检测患者血浆中HLA-Ⅰ类抗体特异性;以流式细胞术检测患者血浆中两性霉素B依赖的血小板抗体。结果 ELISA法检测:血小板特异性抗体和自身抗体均为阴性;Luminex试剂盒测得:患者血清中HLA-Ⅰ类抗体阳性率为1%,未检出有特异性的HLA-Ⅰ类抗体。流式细胞术检测:患者血浆中存在两性霉素B依赖的血小板抗体。结论流式细胞术或可成为检测药物依赖性血小板抗体的实验室标准手段,为临床预防和治疗药物引起的血小板减少症提供可靠的理论和技术保障。
[Abstract]:Objective to establish a standard method for detection of drug-dependent platelet antibodies by flow cytometry. Methods Platelet antibodies were detected by ELISA in a patient with aplastic anemia. The specific and autoantibodies in plasma and platelets were detected by Lifecodes LSATM Class I (Luminex kit. The specificity of HLA-I antibody in plasma was detected by magnetic bead suspension microarray system, and the platelet antibody dependent on amphotericin B in plasma was detected by flow cytometry. Results ELISA assay showed that both platelet specific antibody and autoantibody were negative Luminex test kit. The positive rate of HLA-I antibody in serum was 1 and no specific HLA-I antibody was detected. Flow cytometry: Amphotericin B-dependent platelet antibodies were present in patients' plasma. Conclusion flow cytometry may be a laboratory standard for the detection of drug-dependent platelet antibodies and provide a reliable theoretical and technical guarantee for clinical prevention and treatment of drug-induced thrombocytopenia.
【作者单位】：广州血液中心输血研究所;
【基金】：广东省医学科研基金项目(A2015316) 广州市血液安全重点实验室项目(201509010009) 广东省自然科学基金委(面上项目)(2016A030313123,2016A030313124) 广州市科技计划项目(201607010007);广州市科技计划项目(201707010021)
【分类号】：R556.5

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