替米沙坦对心肌梗死后心力衰竭大鼠左心室非梗死区激活素A及其受体表达的影响

发布时间：2018-07-31 19:31

【摘要】：目的:探讨替米沙坦对心肌梗死后心力衰竭(心衰)大鼠左心室非梗死区激活素A及其受体表达的影响,阐明替米沙坦改善心肌胶原沉积的可能机制。方法:27只Wister大鼠分为假手术组(7只)、模型组(10只)和替米沙坦组(10只)。模型组和替米沙坦组大鼠结扎左冠状动脉前降支,假手术组大鼠左冠状动脉前降支仅穿线不结扎。建模后饲养5周,每组存活6只大鼠。假手术组大鼠给予0.5%CMC(10mL·kg~(-1)),模型组大鼠给予0.5%CMC(10mL·kg~(-1)),替米沙坦组大鼠给予替米沙坦(30mg·kg~(-1));连续灌胃给药4周后测定大鼠全心肥厚指数及左心室肥厚指数,RT-PCR法测定大鼠左心室非梗死区心肌组织中激活素A、激活素Ⅱ型A受体(ActRⅡA)、Ⅱ型B受体(ActRⅡB)、Ⅰ型胶原蛋白(ColⅠ)和Ⅲ型胶原蛋白(ColⅢ)mRNA表达水平以及ColⅠ/ColⅢ比值;免疫组织化学染色观察大鼠左心室非梗死区心肌组织中激活素A蛋白的表达强度。结果:与假手术组比较,模型组大鼠全心肥厚指数和左心室肥厚指数明显升高(P0.01),激活素A、ActRⅡA、ActRⅡB、ColⅠ和ColⅢmRNA表达水平以及ColⅠ/ColⅢ比值升高(P0.01)。免疫组织化学染色,假手术组大鼠非梗死区心肌组织中激活素A蛋白呈低水平表达,模型组大鼠非梗死区心肌组织中激活素A蛋白呈高水平表达。结论:替米沙坦可能通过下调激活素A及其受体的表达水平改善心衰过程中心肌胶原沉积。
[Abstract]:Aim: to investigate the effect of telmisartan on the expression of activin A and its receptor in left ventricular non-infarct area of rats with heart failure after myocardial infarction, and to elucidate the possible mechanism of telmisartan in improving myocardial collagen deposition. Methods 27 Wister rats were divided into sham-operated group (n = 7), model group (n = 10) and telmisartan group (n = 10). The left anterior descending coronary artery was ligated in the model group and telmisartan group, while the left anterior descending branch was not ligated in the sham operation group. After 5 weeks of modeling, 6 rats in each group survived. 0.5%CMC (10mL kg ~ (-1), model group rats were given 0.5%CMC (10mL kg ~ (-1), telmisartan group) rats were given 30mg kg ~ (-1); for 4 weeks, total cardiac hypertrophy index (THR) and left ventricular hypertrophy index (LVH) were measured by RT-PCR. The mRNA expression levels of activin A, ActR 鈪，

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