全氟化碳乳剂对动脉粥样硬化大鼠血管舒缩因子的调控作用

发布时间：2018-08-02 18:36

【摘要】：目的:使用高剪切乳化机制备全氟化碳乳剂(PFCE),通过静脉注射PFCE对动脉粥样硬化(AS)模型大鼠进行干预,观察其内皮功能指标的变化,初步探讨PFCE对AS大鼠血管舒缩活性因子的调控作用及对内皮的可能保护作用。方法:将50只Wistar雄鼠随机分为5组:空白对照组,模型组,PFCE低、中、高剂量组,每组10只。除空白对照组用普通维持饲料外,其余均以维生素D3加高脂饲料喂养诱导实验性大鼠AS模型,PFCE低、中、高剂量组在造模同时给予不同剂量药物干预。12周后,测定各组大鼠血清NO、ET-1、TXA2、6-K-PG的含量,苏木精-伊红染色法观察各组大鼠主动脉病理变化。结果:模型组NO、TXA2含量显著降低,ET-1、6-K-PG含量显著升高,与空白对照组比较差异有统计学意义(P0.05),且NO/ET-1比值、TXA2/6-K-PG比值平衡失调;PFCE高、中剂量组较模型组血清NO、TXA2含量显著升高,ET-1、6-K-PG含量显著降低(P0.05),并改善NO/ET-1及TXA2/6-K-PG的比值(P0.05);PFCE低剂量组与模型组相比血清中各指标差异无统计学意义(P0.05);PFCE高剂量组与PFCE低剂量组相比血清NO、TXA2含量显著升高,ET-1、6-K-PG含量显著降低(P0.05),与中等剂量组差异不显著(P0.05);PFCE高、中剂量组较PFCE低剂量组减轻AS大鼠主动脉组织形态学变化更明显。结论:中等剂量以上PFCE可能通过调控AS大鼠血管舒缩活性因子,起到保护血管内皮的功能,发挥阻止AS进一步发生和发展的作用。
[Abstract]:Objective: to observe the changes of endothelial function in atherosclerotic (AS) model rats by intravenous injection of (PFCE), a perfluorocarbon emulsion prepared by high shear emulsifying machine. Objective: to investigate the regulatory effect of PFCE on vasomotor activity factor and its possible protective effect on endothelium in as rats. Methods: fifty male Wistar rats were randomly divided into 5 groups: blank control group, model group, low, medium and high dose groups with 10 rats in each group. With the exception of the blank control group, the rats were fed with vitamin D3 plus high fat diet to induce experimental as model with low PFCE. The middle and high dose groups were treated with different doses of drugs at the same time for 12 weeks. The contents of TXA2 6-K-PG and the pathological changes of aorta in each group were observed by hematoxylin and eosin staining. Results: the content of NOTXA2 in the model group was significantly lower than that in the control group, and the content of 6-K-PG was significantly higher than that of the control group (P0.05), and the ratio of NO/ET-1 to TXA _ 2 / 6-K-PG was not balanced significantly (P > 0.05), and the ratio of TXA _ 2 / 6-K-PG was higher than that of the control group. Compared with the model group, the serum NOTXA2 content in the middle dose group was significantly increased (P0.05), and the ratio of NO/ET-1 to TXA2/6-K-PG was improved (P0.05). There was no significant difference in serum indexes between the low dose group and the model group (P0.05). Compared with the middle dose group, the content of TXA2 in serum increased significantly (P0.05), the content of 6-K-PG decreased significantly (P0.05), but the level of PFCE was higher than that in the middle dose group (P0.05). The changes of aortic histomorphology in as rats were significantly reduced in middle dose group than in low dose PFCE group. Conclusion: the medium dose of PFCE may protect the endothelium and prevent the further development of as by regulating vasomotor activity factor in as rats.
【作者单位】：青岛大学医学院心内科;中国人民解放军401医院心内科;
【分类号】：R543.5

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