IL-6介导的早期炎症促进新生小鼠心肌细胞增殖

发布时间：2018-08-06 18:02

【摘要】：目的探索IL-6介导的早期炎症在新生小鼠心脏再生中的作用及其机制。方法新生1 d的小鼠随机分为模型组和假手术组,前者低温麻醉切除心尖成模,后者只作开胸缝合。术后1、3、7和21 d摘取心脏。石蜡切片后HE染色,q PCR检测增殖相关基因表达;并检测炎症因子基因il-6、il-1β及icam1转录水平的变化。ELISA检测血清中IL-6、TNF-α及IL-1β的含量,Western blot检测IL-6下游底物Akt、Akt(S473)、Stat3、Stat3(T705)的表达量,并检测Akt和Stat3磷酸化负向调控基因PP2A的变化。结果术后7 d模型组小鼠基本完成心脏再生过程,切片染色显示再生心脏的心尖结构完整,心肌细胞增殖相关基因表达增加。IL-6、TNF-α及IL-1β等炎症因子在术后早期急剧升高,其下游底物Akt和Stat3磷酸化水平显著升高,PP2A及其亚基在小鼠心脏再生过程中显著降低。结论新生1 d的小鼠具备完全的心脏再生能力,心尖切除手术会诱导炎症反应增加,IL-6介导的早期炎症促进了新生小鼠心肌细胞的增殖。
[Abstract]:Objective to explore the role and mechanism of IL-6-mediated early inflammation in cardiac regeneration in newborn mice. Methods one day old mice were randomly divided into two groups: model group and sham operation group. The heart was excised on 7 and 21 days after operation. After paraffin section, the expression of proliferation-related genes was detected by HE staining, and the expression of IL-6il-1 尾 and icam1 was detected by HE staining. The levels of IL-6TNF- 伪 and IL-1 尾 in serum were detected by Elisa and the expression of Akt (S473) -Stat3Stat3 (T705) was detected by Western blot. The changes of Akt and Stat3 phosphorylation negative regulatory gene PP2A were detected. Results on the 7th day after operation, the cardiac regeneration process was basically completed in the model group. Section staining showed that the apical structure of the regenerated heart was intact, and the expression of proliferation-related genes in cardiomyocytes was increased. The inflammatory factors such as IL-6 TNF- 伪 and IL-1 尾 increased sharply in the early postoperative period. The phosphorylation levels of Akt and Stat3 in the downstream substrates increased significantly, and PP2A and its subunits decreased significantly during cardiac regeneration in mice. Conclusion the neonatal mice have the ability to regenerate the heart completely. Apical resection can induce inflammation and increase the early inflammation mediated by IL-6, which can promote the proliferation of cardiac myocytes in newborn mice.
【作者单位】：泰兴市人民医院心内科;
【基金】：泰兴市人民医院重点项目(try1504)
【分类号】：R54

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