辛伐他汀对快速起搏早期家兔心房肌超微结构的影响及可能机制

发布时间：2018-08-13 08:52

【摘要】：目的建立家兔快速心房起搏模型,探讨辛伐他汀对快速心房起搏早期心房超微结构及L型钙通道α1c蛋白的影响。方法 30只家兔随机分为对照组、心房快速起搏组、辛伐他汀组各10只。辛伐他汀组5 mg·kg~(-1)·d~(-1)辛伐他汀灌胃2 w,其余组以等量生理盐水灌胃,对照组不行起搏,心房快速起搏组和辛伐他汀组行快速心房起搏(800次/min)建立家兔快速起搏模型,急性起搏8 h后取右房组织,电镜观察心房肌超微结构的改变,免疫组化法观察急性起搏8 h后右房组织L型钙通道α1c蛋白表达。结果心房快速起搏组起搏8 h后心房肌细胞超微结构有明显变化,如肌原纤维排列紊乱、肌小节减少,肌溶解、线粒体肿胀、空泡样改变、糖原聚集等心房结构重构的改变,心房肌细胞L型钙通道的α1c亚单位的蛋白表达水平较对照组明显下降。辛伐他汀组心房肌超微结构的改变相对较轻,L型钙通道α1c亚单位的蛋白表达水平较对照组下降不明显。结论辛伐他汀可明显改善快速起搏早期家兔心房肌超微结构的变化,对快速心房起搏导致的L型钙通道α1c亚单位的表达有一定保护作用。
[Abstract]:Objective to establish a rabbit model of rapid atrial pacing and to investigate the effects of simvastatin on atrial ultrastructure and 伪 1c protein of L-type calcium channel in the early stage of rapid atrial pacing. Methods Thirty rabbits were randomly divided into control group, atrial rapid pacing group and simvastatin group. Simvastatin group (5 mg kg ~ (-1) d ~ (-1) was intragastrically perfused with simvastatin for 2 weeks. The other groups were fed with the same amount of normal saline for 2 weeks, but the control group was not. The rapid atrial pacing group and simvastatin group were treated with rapid atrial pacing (800 times / min) to establish a rabbit model of rapid atrial pacing. The changes of ultrastructure of atrial muscle were observed by electron microscope and the expression of L-type calcium channel 伪 1c protein was observed by immunohistochemical method after 8 h acute pacing. Results in the rapid atrial pacing group, the ultrastructure of atrial myocytes changed significantly after 8 h pacing, such as the disorder of myofibril arrangement, the decrease of muscle section, myolysis, swelling of mitochondria, vacuolar changes, glycogen aggregation and other changes of atrial structural remodeling. The protein expression of 伪 1c subunit of L-type calcium channel in atrial myocytes was significantly lower than that in control group. The expression of 伪 1c subunit of L-type calcium channel in simvastatin group was not significantly lower than that in control group. Conclusion Simvastatin can obviously improve the ultrastructural changes of rabbit atrial muscle in the early stage of rapid pacing, and has a protective effect on the expression of 伪 1c subunit of L-type calcium channel induced by rapid atrial pacing.
【作者单位】：中国人民解放军第105医院心内二科;
【基金】：南京军区联勤部资助课题(No.11YG04)
【分类号】：R541.75

【相似文献】