透明质酸对游离脂肪酸引起的人主动脉血管平滑肌细胞表达IL-6、IL-8和TNF-α的影响

发布时间：2018-08-16 18:05

【摘要】：目的探讨透明质酸(HA)对游离脂肪酸(FFA)引起的人主动脉血管平滑肌细胞(HA-VSMC)表达白细胞介素6(IL-6)、IL-8和肿瘤坏死因子α(TNF-α)的影响。方法培养HA-VSMC,用不同浓度(0、100、200、400μmol/L)FFA和不同浓度FFA+高分子量HA(HMW-HA)或低分子量HA(LMW-HA)处理细胞24、48 h。噻唑蓝法检测FFA和FFA+HA对HA-VSMC存活率的影响;Ed U染色检测细胞增殖活性;酶联免疫吸附法检测培养上清IL-6、IL-8和TNF-α含量;实时荧光定量PCR和Western blot检测IL-6、IL-8和TNF-αmRNA及蛋白表达水平。结果(1)FFA在400μmol/L内呈浓度依赖性诱导HA-VSMC增殖;(2)HMW-HA、LMW-HA均对FFA诱导的HA-VSMC增殖有抑制作用,且HMW-HA抑制作用强于LMW-HA;(3)FFA显著促进HA-VSMC IL-6、IL-8和TNF-α的分泌,且有明显的时间和浓度依耐性;HA可以抑制上述作用;(4)实时荧光定量PCR和Western blot结果显示,FFA+HMWHA组、FFA+LMW-HA组IL-6、IL-8、TNF-αmRNA及蛋白表达水平均低于FFA组(均P0.01),其中FFA+HMW-HA组又低于FFA+LMW-HA组(P0.01)。结论 (1)FFA诱导HA-VSMC IL-6、IL-8、TNF-αmRNA和蛋白表达升高;(2)HA对FFA引起的HA-VSMC IL-6、IL-8和TNF-α的表达有抑制作用,且HMW-HA抑制效果强于LMW-HA。
[Abstract]:Objective to investigate the effect of hyaluronic acid (HA) on the expression of interleukin-6 (IL-6) IL-8 and tumor necrosis factor- 伪 (TNF- 伪) in human aortic vascular smooth muscle cells (HA-VSMC) induced by free fatty acid (FFA). Methods HA-VSMC was cultured and treated with different concentration (0100200400 渭 mol/L) FFA and FFA high molecular weight HA (HMW-HA) or low molecular weight HA (LMW-HA) for 24 h. The effect of FFA and FFA HA on the survival rate of HA-VSMC was detected by thiazolyl blue method. The proliferative activity was detected by Ed U staining, the IL-8 and TNF- 伪 contents in supernatants were detected by enzyme-linked immunosorbent assay (Elisa), the IL-8 and TNF- 伪 mRNA and protein expression levels were detected by real-time fluorescence quantitative PCR and Western blot. Results (1) FFA induced the proliferation of HA-VSMC in a concentration-dependent manner, (2) HMW-HALMW-HA inhibited the proliferation of HA-VSMC induced by FFA, and the inhibitory effect of HMW-HA was stronger than that of LMW-HA.The (3) FFA significantly promoted the secretion of IL-8 and TNF- 伪 in HA-VSMC IL-6. The results of real-time fluorescence quantitative PCR and Western blot showed that the expression levels of IL-6 and IL-8 TNF- 伪 mRNA and protein in FFA LMW-HA group were lower than those in FFA group (P0.01), and FFA HMW-HA group was lower than FFA LMW-HA group (P0.01). Conclusion (1) FFA induces an increase in the expression of TNF- 伪 mRNA and protein in HA-VSMC IL-6 and IL-8, and (2) HA can inhibit the expression of IL-8 and TNF- 伪 in HA-VSMC induced by FFA, and the inhibitory effect of HMW-HA is stronger than that of LMW-HA.
【作者单位】：宁夏医科大学临床医学院检验系;甘肃省人民医院中心实验室;甘肃省人民医院内分泌科甘肃省内分泌代谢病重点实验室;甘肃省人民医院临床检验中心;
【分类号】：R363

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