Syndecan-1对大鼠心肌成纤维细胞生物学行为影响
发布时间：2018-08-17 15:47
【摘要】：目的评估syndecan-1(Sdc1)对大鼠心肌成纤维细胞生物学行为的影响,为心肌梗死(MI)后心室重构的防治提供依据。方法体外分离培养SD大鼠乳鼠心肌成纤维细胞,分别转染Sdc1 siRNA质粒和Sdc1高表达质粒,并设立空白对照组、无关序列和空载质粒作为阴性对照。采用流式细胞术检测细胞增殖,CCK-8法检测细胞活力,酶联免疫法(ELISA)检测胶原(羟脯氨酸)分泌能力及Transwell细胞迁移实验检测细胞迁移能力。结果Sdc1 siRNA组心肌成纤维细胞S期比例、增殖指数、细胞活力、胶原(羟脯氨酸)分泌量和迁移细胞数分别为(8.36±0.23)%、(13.2±0.5)%、(1.72±0.12)、(1162.3±60.4)pg/mL和170个/孔,明显高于空白对照和阴性对照组(P0.01);Sdc1高表达质粒组心肌成纤维细胞S期比例、增殖指数、细胞活力、胶原(羟脯氨酸)分泌量和迁移细胞数分别为(6.48±0.22)%、(10.8±0.6)%、(1.30±0.11)、(346.8±52.1)pg/mL和50个/孔,明显低于空白对照和空载质粒组(P0.01)。结论 Sdc1能够抑制心肌成纤维细胞的增殖、活力、迁移和分泌能力。
[Abstract]:Objective to evaluate the effects of syndecan-1 (Sdc1) on the biological behavior of cardiac fibroblasts in rats and to provide evidence for the prevention and treatment of ventricular remodeling after myocardial infarction (MI). Methods SD rat myocardial fibroblasts were isolated and cultured in vitro and transfected with Sdc1 siRNA plasmid and Sdc1 high expression plasmid respectively. The blank control group was set up and the unrelated sequence and empty plasmid were used as negative control. The cell viability was detected by flow cytometry (FCM) and CCK-8 assay. The activity of collagen (hydroxyproline) was detected by enzyme-linked immunosorbent assay (ELISA) and the migration ability of Transwell cells was detected by Transwell cell migration assay. Results in Sdc1 siRNA group, the ratio of S phase, proliferation index, cell viability, secretion of collagen (hydroxyproline) and the number of migrating cells were (8.36 卤0.23), (13.2 卤0.5), (1.72 卤0.12), () 1162.3 卤60.4 pg/mL and 170 / well, respectively. The ratio of S phase, proliferation index, cell viability, collagen (hydroxyproline) secretion and the number of migrating cells were (6.48 卤0.22), (10.8 卤0.60), (1.30 卤0.11), (卤52.1) pg/mL and (50 / well), respectively. It was significantly lower than the blank control group and the blank plasmid group (P0.01). Conclusion Sdc1 can inhibit the proliferation, activity, migration and secretion of myocardial fibroblasts.
【作者单位】： 中山大学孙逸仙纪念医院;
【基金】：广东省科技计划项目(2013B021800096)
【分类号】：R542.22
，
本文编号：2188128
[Abstract]:Objective to evaluate the effects of syndecan-1 (Sdc1) on the biological behavior of cardiac fibroblasts in rats and to provide evidence for the prevention and treatment of ventricular remodeling after myocardial infarction (MI). Methods SD rat myocardial fibroblasts were isolated and cultured in vitro and transfected with Sdc1 siRNA plasmid and Sdc1 high expression plasmid respectively. The blank control group was set up and the unrelated sequence and empty plasmid were used as negative control. The cell viability was detected by flow cytometry (FCM) and CCK-8 assay. The activity of collagen (hydroxyproline) was detected by enzyme-linked immunosorbent assay (ELISA) and the migration ability of Transwell cells was detected by Transwell cell migration assay. Results in Sdc1 siRNA group, the ratio of S phase, proliferation index, cell viability, secretion of collagen (hydroxyproline) and the number of migrating cells were (8.36 卤0.23), (13.2 卤0.5), (1.72 卤0.12), () 1162.3 卤60.4 pg/mL and 170 / well, respectively. The ratio of S phase, proliferation index, cell viability, collagen (hydroxyproline) secretion and the number of migrating cells were (6.48 卤0.22), (10.8 卤0.60), (1.30 卤0.11), (卤52.1) pg/mL and (50 / well), respectively. It was significantly lower than the blank control group and the blank plasmid group (P0.01). Conclusion Sdc1 can inhibit the proliferation, activity, migration and secretion of myocardial fibroblasts.
【作者单位】： 中山大学孙逸仙纪念医院;
【基金】：广东省科技计划项目(2013B021800096)
【分类号】：R542.22
，
本文编号：2188128
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