血清长链非编码脱氧核糖核酸KCNQ1重叠转录物1水平与冠状动脉病变的相关性研究

发布时间：2018-08-28 20:13

【摘要】：目的:探讨检测长链非编码脱氧核糖核酸(RNA)KCNQ1重叠转录物1(KCNQ1OT1)在冠状动脉粥样硬化性心脏病(冠心病)患者血清中的表达及其临床意义。方法:纳入我院收治的196例患者,98例冠心病患者为冠心病组,98例非冠心病患者为对照组。用实时荧光定量多聚酶链反应(q RT-PCR)检测血清KCNQ1OT1及P53基因的表达水平,分析KCNQ1OT1与血清P53基因表达水平及临床病例特征的相关性。结果:与对照组比较,冠心病组患者血清KCNQ1OT1的表达明显增高,差异具有统计学意义(P0.05)。P53基因在冠心病组患者血清中的表达水平明显低于对照组(P0.05)。线性相关分析发现血清KCNQ1OT1与P53基因的表达呈负相关关系(r=-0.856,P0.001)。多因素Logistic回归分析发现血清KCNQ1OT1的表达与冠心病存在独立相关(P0.05)。结论 :冠心病患者血清KCNQ1OT1表达显著增加,并存在独立相关。
[Abstract]:Aim: to investigate the expression and clinical significance of (RNA) KCNQ1 overlap transcription 1 (KCNQ1OT1) in patients with coronary atherosclerotic heart disease (CHD). Methods: a total of 196 patients with coronary heart disease (CHD) were included in our hospital. 98 patients with coronary heart disease (CHD) were treated as control group (n = 98). Real-time fluorescence quantitative polymerase chain reaction (Q RT-PCR) was used to detect the expression of serum KCNQ1OT1 and p53 gene, and the correlation between KCNQ1OT1 and serum p53 gene expression and clinical features was analyzed. Results: compared with the control group, the expression of serum KCNQ1OT1 in CHD group was significantly higher than that in control group (P0.05). The expression level of p53 gene in serum of CHD group was significantly lower than that of control group (P0.05). Linear correlation analysis showed a negative correlation between serum KCNQ1OT1 and p53 gene expression (r-0. 856% P 0. 001). Multivariate Logistic regression analysis showed that there was an independent correlation between serum KCNQ1OT1 expression and coronary heart disease (P0.05). Conclusion: the expression of serum KCNQ1OT1 in patients with coronary heart disease is significantly increased, and there is an independent correlation.
【作者单位】：成都大学附属医院心内科;
【分类号】：R541.4

【相似文献】