MMP-9在老年风心病心房纤颤患者心房纤维化的表达及临床意义
发布时间：2018-08-30 14:11
【摘要】：目的:观察MMP-9在老年风湿性心脏病(风心病)心房纤颤(房颤)患者心房纤维化的表达,探讨MMP-9与老年风心病房颤患者心房纤维化的关系。方法:选取西安交通大学第一附属院、西京医院、陕西省人民医院心脏外科2012年5月28日至2012年8月13日因风湿性心脏瓣膜病住院进行心脏瓣膜置换手术的患者32例,根据有无房颤分为两组:慢性房颤组16例;窦性心律组16例。收集上述患者基本临床资料,如年龄、性别、血压、现病史、心电图及心脏彩超等资料。所有患者于心脏外科手术中建立体外循环心脏停跳前,在体外循环插管前及右心房打开后获取右心房组织(0.3-0.5cm3),采用HE染色、Masson染色观察两组心肌纤维化程度,采用实时荧光定量多聚核苷酸链式应反技术(RT-q PCR)检测心房组织中Ⅰ、Ⅲ型胶原的m RNA水平,采用免疫印迹试验(Western blotting)测定心房组织MMP-9的表达。结果:(1)HE染色结果显示与窦性心律组患者相比,房颤组患者心房肌纤维增粗、排列松散不齐,可见明显纤维组织增生。Masson染色结果显示与窦性心率组患者相比,房颤组患者心房组织中出现粗细不均的蓝色胶原纤维组织,心肌间质胶原沉积显著增多;房颤组心房组织胶原容积分数显著高于窦性心律组,组间比较有统计学差异(P0.05)。(2)房颤组心房肌组织标本中Ⅰ型胶原的m RNA相对表达明显高于窦性心率组;心房肌组织标本中Ⅲ型胶原的m RNA相对表达虽然在房颤组中较窦性心律组增高,但组间比较无统计学差异。(3)房颤组心房组织MMP-9蛋白表达水平高于窦性心律组(P0.05);房颤组MMP-9蛋白表达水平与I型胶原m RNA、左和右房内径均呈明显正相关(r分别为0.511、0.534、0.736,均P0.05)。结论:老年风心病房颤患者心房组织中MMP-9的蛋白表达变化是房颤患者心房纤维化的分子机制之一,与房颤的发生与维持有关。
[Abstract]:Aim: to observe the expression of MMP-9 in atrial fibrillation (AF) in elderly patients with rheumatic heart disease (RHD) and to investigate the relationship between MMP-9 and atrial fibrosis in elderly patients with rheumatic heart disease (RHD). Methods: from May 28, 2012 to August 13, 2012, 32 patients with rheumatic valvular valve replacement were selected from the first affiliated Hospital of Xi'an Jiaotong University, Xijing Hospital and Shaanxi Provincial people's Hospital. The patients were divided into two groups according to atrial fibrillation: chronic atrial fibrillation group (16 cases) and sinus rhythm group (16 cases). Basic clinical data such as age, sex, blood pressure, current history, electrocardiogram and echocardiography were collected. Before cardiopulmonary bypass (CPB) cardiac arrest was established in all patients, right atrial tissue (0.3-0.5cm3) was obtained before CPB catheterization and right atrium was opened. The degree of myocardial fibrosis was observed by HE staining and Masson staining. Real-time fluorescence quantitative polynucleotide chain reaction (RT-q PCR) technique was used to detect the m RNA level of type 鈪，
本文编号：2213310
[Abstract]:Aim: to observe the expression of MMP-9 in atrial fibrillation (AF) in elderly patients with rheumatic heart disease (RHD) and to investigate the relationship between MMP-9 and atrial fibrosis in elderly patients with rheumatic heart disease (RHD). Methods: from May 28, 2012 to August 13, 2012, 32 patients with rheumatic valvular valve replacement were selected from the first affiliated Hospital of Xi'an Jiaotong University, Xijing Hospital and Shaanxi Provincial people's Hospital. The patients were divided into two groups according to atrial fibrillation: chronic atrial fibrillation group (16 cases) and sinus rhythm group (16 cases). Basic clinical data such as age, sex, blood pressure, current history, electrocardiogram and echocardiography were collected. Before cardiopulmonary bypass (CPB) cardiac arrest was established in all patients, right atrial tissue (0.3-0.5cm3) was obtained before CPB catheterization and right atrium was opened. The degree of myocardial fibrosis was observed by HE staining and Masson staining. Real-time fluorescence quantitative polynucleotide chain reaction (RT-q PCR) technique was used to detect the m RNA level of type 鈪，
本文编号：2213310
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