间歇性碱刺激对高磷诱导的大鼠血管环钙化的影响及可能机制

发布时间：2018-09-01 14:52

【摘要】：目的探讨间歇性碱刺激对高磷诱导的大鼠胸主动脉血管环钙化的影响及可能机制。方法体外分离大鼠胸主动脉血管环,将其随机分为正常对照组、高磷组(含10 mmol/Lβ-甘油磷酸的培养基)和间歇性碱刺激组(在高磷培养基的基础上调整pH值为7.7)。干预14天后,采用免疫组织化学方法检测L型钙通道(LTCC)β3亚基、Runt相关转录因子2(Runx2)和平滑肌蛋白22α(SM22α)的表达情况;采用硝酸银染色法和钙含量测定法检测血管环钙化程度。结果与正常对照组相比,高磷组的钙含量、Runx2和LTCCβ3亚基表达水平均增加(P0.001);与高磷组相比,间歇性碱刺激组的钙含量、Runx2和LTCCβ3亚基表达水平均有显著增加(P0.001)。同时,高磷组棕黑色钙化结节较正常对照组增多,间歇性碱刺激组棕黑色钙化结节较高磷组增多。与正常对照组相比,高磷组的SM22α表达水平下降(P0.001);与高磷组相比,间歇性碱刺激组的SM22α表达水平显著下降(P0.001)。相关性分析显示,LTCCβ3亚基与Runx2蛋白表达呈正相关(r=0.740,P=0.002),与SM22α蛋白表达呈负相关(r=-0.670,P=0.006)。结论间歇性碱刺激可以促进高磷诱导的大鼠胸主动脉血管环钙化,其可能通过上调LTCCβ3亚基表达,促进血管平滑肌细胞向成骨/成软骨表型转化,进而促进血管环钙化的发生。
[Abstract]:Objective to investigate the effect and possible mechanism of intermittent alkali stimulation on calcification of thoracic aortic rings induced by high phosphorus in rats. Methods the aortic rings of thoracic aorta of rats were isolated in vitro and randomly divided into normal control group, high phosphorus group (medium containing 10 mmol/L 尾 -glycerophosphoric acid) and intermittent alkali stimulation group (adjusting pH value to 7. 7 on the basis of high phosphorus medium). After 14 days of intervention, the expression of (LTCC) 尾 3 subunit (LTCC) 尾 3 related transcription factor 2 (Runx2) and smooth muscle protein 22 伪 (SM22 伪) in L-type calcium channel were detected by immunohistochemistry, and the calcification degree of vascular rings was detected by silver nitrate staining and calcium content measurement. Results compared with the control group, the calcium content and the expression level of LTCC 尾 3 subunit in high phosphorus group were increased (P0.001), and the calcium content and LTCC 尾 3 subunit expression level in intermittent alkali stimulation group were significantly higher than those in high phosphorus group (P0.001). At the same time, the number of brown-black calcified nodules in high-phosphorus group was higher than that in normal control group, and the number of brown-black calcified nodules in intermittent alkali stimulation group was higher than that in high-phosphorus group. Compared with the normal control group, the expression of SM22 伪 in the high phosphorus group was decreased (P0. 001), and the SM22 伪 expression level in the intermittent alkaline stimulation group was significantly lower than that in the high phosphorus group (P0. 001). The correlation analysis showed that the expression of LTCC 尾 3 subunit was positively correlated with the expression of Runx2 protein (r = 0.740) and negatively correlated with the expression of SM22 伪 protein (r ~ (-0.670) P ~ (0. 006). Conclusion intermittent alkali stimulation can promote calcification of aortic rings in rats induced by high phosphorus, which may promote the transformation of vascular smooth muscle cells to osteoblast / chondrogenic phenotype by upregulating the expression of LTCC 尾 3 subunit, and then promote the occurrence of vascular ring calcification.
【作者单位】：河北医科大学第四医院肾内科;
【基金】：河北省自然科学基金项目(2012206157) 河北省科技计划项目(16397733D、20150351、20150310)
【分类号】：R54;R692.5

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