伊洛前列素对低氧性肺动脉高压大鼠肺部内皮素-1表达的影响

发布时间：2018-09-17 13:27

【摘要】：目的探讨伊洛前列素对低氧性肺动脉高压(HPH)大鼠肺部内皮素-1(ET-1)的影响。方法雄性SD大鼠32只,分为正常(NC)组,正常治疗(NT)组,低氧(HC))组,低氧治疗(HT)组,每组8只。NC组和NT组在常氧条件下饲养3周;HC和HT组放置于低氧舱(O2浓度10%)进行缺氧处理3周(8h/d);NT和HT组每天进行伊洛前列素雾化吸入治疗(2μg/kg)。第3周结束时,测量各组平均肺动脉压(mPAP),右心室收缩期末压力(RVSP),右心室肥厚指数。HE染色观察各组肺动脉形态学变化,计算肺小动脉的血管壁厚百分比(WT%),RT-PCR分析各组肺组织匀浆中ET-1的变化趋势。结果 HC组大鼠血流动力学指标较其他组明显升高,HT组相关指标表达与NC组比较差异无统计学意义(P0.05)。HC组较其他组肺动脉管壁增厚、管腔狭窄明显;HT组则有不同程度逆转。HC组大鼠肺组织匀浆中ET-1表达较NC组明显升高,HT组中ET-1表达与NC组比较差异无统计学意义(P0.05)。结论伊洛前列素对HPH有明显的治疗作用,可以降低HPH大鼠肺部ET-1的释放,阻止肺血管重塑。
[Abstract]:Objective to investigate the effect of iloprostol on pulmonary endothelin-1 (ET-1) in hypoxic pulmonary hypertension (HPH) rats. Methods Thirty-two male SD rats were divided into normal (NC) group, normal (NT) group, hypoxic (HC) group and hypoxic (HT) group. Each group (n = 8) and NT group (n = 8) were fed under normoxic conditions for 3 weeks. HC and HT groups were placed in hypoxic chamber (O _ 2 concentration 10%) for 3 weeks after anoxic treatment (8h/d) and NT and HT groups were treated with iloprostol atomization inhalation (2 渭 g/kg) every day. At the end of the third week, mean pulmonary artery pressure (mPAP), right ventricular end-systolic pressure (RVSP), right ventricular hypertrophy index (RVH), and HE staining were measured to observe the morphologic changes of pulmonary artery in each group. The vascular wall thickness percentage (WT%) of pulmonary arterioles was calculated. RT-PCR was used to analyze the change trend of ET-1 in lung homogenate of each group. Results the expression of hemodynamic indexes in HC group was significantly higher than that in other groups. There was no significant difference between NC group and NC group (P0.05). The thickness of pulmonary artery wall in HC group was thicker than that in other groups. The expression of ET-1 in lung homogenate of HC group was significantly higher than that of NC group (P0.05). Conclusion iloprostol has obvious therapeutic effect on HPH, which can reduce the release of ET-1 and prevent pulmonary vascular remodeling in HPH rats.
【作者单位】：三峡大学呼吸病研究所/湖北省宜昌市中心人民医院呼吸内科;
【基金】：湖北省自然科学基金资助项目(2012FFB06301);湖北省自然科学基金指导性计划项目(2014CFC1037)
【分类号】：R544.1

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