金属硫蛋白基因G-201A多态性与原发性高血压的相关性

发布时间：2018-10-08 14:32

【摘要】：目的探讨金属硫蛋白(MT)基因家族中的MT4基因G-201A位点单核苷酸多态性(SNPs)与云南地区汉族人群中原发性高血压的相关性。方法选取原发性高血压患者301例,正常对照组301例。应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析技术检测MT4基因G-201A位点的基因多态性。结果原发性高血压组、正常对照组MT4 G-201A基因型GG、GA、AA的频率分别为42.2%、48.5%、9.3%;42.5%、51.5%、6.0%;基因型分布均符合Hardy-Weinberg平衡(P0.05)。MT4 G-201A多态性:原发性高血压组和正常对照组比较分布无显著差异(P0.05);等位基因频率分布无显著差异(P0.05)。两组男性基因型及等位基因频率分布均有统计学差异(P0.05);回归分析示年龄、性别、体重指数(BMI)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、总胆固醇(TC)、尿酸(UA)与原发性高血压的发生相关(P0.05),等位基因A携带者(GA+AA型)与原发性高血压的发生无相关性(P0.05)。原发性高血压组与正常对照组比较,年龄、BMI、甘油三酯(TG)、HDL-C、LDLC、UA有统计学差异(P0.05)。原发性高血压组中GG组与AA组比较,性别、TC有统计学差异(P0.05)。结论 MT基因G-210A多态性与原发性高血压无相关性;等位基因A携带者与原发性高血压的发生无相关性;MT基因G-201A多态性与脂代谢相关,GG组有较高的TC。
[Abstract]:Objective to investigate the association of G-201A polymorphism (SNPs) of MT4 gene in metallothionein (MT) gene family with essential hypertension in Yunnan Han population. Methods 301 patients with essential hypertension and 301 normal controls were selected. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the G-201A locus polymorphism of MT4 gene. Results essential hypertension group, The frequencies of GG,GA,AA of MT4 G-201A genotype in normal control group were 42.2and 48.55.The genotype distribution was consistent with Hardy-Weinberg balance (P0.05). MT4 G-201A polymorphism: there was no significant difference between essential hypertension group and normal control group (P0.05); there was no significant difference in allele frequency distribution (P0.05). The frequency distribution of genotype and allele was significantly different between the two groups (P0.05). Body mass index (BMI),) high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), total cholesterol (TC), uric acid (UA) were associated with essential hypertension (P0.05), allele A carrier (GA AA had no correlation with the occurrence of essential hypertension (P0.05). Compared with the control group, the age of BMI and triglyceride (TG) were significantly different between the essential hypertension group and the normal control group (P0.05). In essential hypertension group, there was statistical difference between GG group and AA group (P0.05). Conclusion there is no correlation between G-210A polymorphism of MT gene and essential hypertension, and there is no correlation between G-201A polymorphism of MT gene and GG group of essential hypertension. There is a higher TC. in GG group associated with lipid metabolism.
【作者单位】：昆明医科大学第一附属医院内分泌科;郑州市中心医院内分泌科;昆明医科大学第一附属医院检验科;
【基金】：国家自然科学基金地区科学基金项目(81660153) 云南省应用基础研究(昆医联合专项)[2017FE468(-197)] 云南省卫生厅内设机构项目(No.2012WS0030)
【分类号】：R544.11

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