胰升血糖素样肽1对晚期氧化蛋白产物诱导的心肌细胞凋亡保护作用的研究

发布时间：2018-10-09 15:29

【摘要】：目的探讨胰升血糖素样肽1(GLP-1)对晚期氧化蛋白产物(AOPPs)诱导的心肌细胞凋亡的保护作用及机制。方法以H9C2心肌细胞为研究对象,用空白对照、RSA对照、AOPPs、GLP-1、AOPPs+GLP-1及AOPPs+GLP-1+LY294002分别干预细胞24 h。采用cck-8法检测细胞的增殖活力DCFH-DA荧光探针检测细胞内活性氧的产生;Annexin V-FITC/PI双染色法检测细胞凋亡;Western blot检测细胞内p-Akt、p-Bad、Bcl-2、Bax、active-caspase-3蛋白的表达。结果 GLP-1抵抗AOPPs引起的细胞增殖活力下降[(1.409±0.099)vs(0.929±0.083),P0.01],减少AOPPs诱导的活性氧簇产生[(47.817±0.878)%vs(25.413±2.597)%,P0.01]及细胞凋亡[(15.773±3.130)%vs(9.715±0.757)%,P0.05];GLP-1能恢复被AOPPs抑制的Akt及Bad的磷酸化,增加细胞抗凋亡蛋白Bcl-2、减少促凋亡蛋白Bax及凋亡执行子active-caspase-3的表达。结论 GLP-1通过PI3K/Akt/Bad信号通路改变促凋亡及抗凋亡蛋白间的平衡,保护AOPPs诱导的H9C2心肌细胞凋亡。
[Abstract]:Objective to investigate the protective effect and mechanism of glucagon like peptide 1 (GLP-1) on cardiomyocyte apoptosis induced by late oxidative protein product (AOPPs). Methods H9C2 cardiomyocytes were treated with AOPPs1 GLP-1AOPPs GLP-1 and AOPPs GLP-1 LY294002 for 24 h. Cck-8 assay was used to detect the proliferation activity of cells. DCFH-DA fluorescence probe was used to detect the production of reactive oxygen species (Ros) in cells. Annexin V-FITC/PI double staining method was used to detect apoptosis. Western blot was used to detect the expression of p-Aktna-p-Bad-Bcl-2Bax-active-caspase-3 protein. Results GLP-1 decreased the cell proliferation activity induced by AOPPs [(1.409 卤0.099) vs (0.929 卤0.083) P0.01], decreased the production of reactive oxygen species induced by AOPPs [(47.817 卤0.878) vs (25.413 卤2.597) P 0.01] and apoptosis [(15.773 卤3.130) vs (9.715 卤0.757)] GLP-1 could restore the phosphorylation of Akt and Bad inhibited by AOPPs. The expression of pro-apoptotic protein Bax and apoptotic executor active-caspase-3 was decreased by increasing the expression of anti-apoptotic protein Bcl-2,. Conclusion GLP-1 changes the balance between pro-apoptosis and anti-apoptotic proteins through PI3K/Akt/Bad signaling pathway and protects H9C2 cardiomyocytes from apoptosis induced by AOPPs.
【作者单位】：广东省韶关市粤北人民医院内分泌科;
【分类号】：R542.2;R587.2

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