尼可地尔后适应对猪心脏骤停后心脑肾缺血再灌注损伤的保护作用

发布时间：2018-10-09 18:17

【摘要】：目的探讨猪心脏骤停模型中尼可地尔后适应对心脑肾缺血再灌注损伤的作用。方法 22头长白猪随机分为假手术组(n=4)和复苏组(n=18)。复苏组在手术后给予电刺激诱导室颤,4 min后行心肺复苏术。假手术组给予手术而不诱发室颤。复苏组16头长白猪复苏成功并随机分为盐水对照组(n=8)和尼可地尔组(n=8),尼可地尔组即刻经中央静脉注射尼可地尔(150μg/kg),随后以3μg/(kg·min)静滴至再灌注结束;盐水对照组给予同剂量生理盐水。致颤前和自主循环恢复后5 min、30 min、3 h、6 h采取静脉血以测定心肌肌钙蛋白I(c Tn I)水平。自主循环恢复后6 h速取其左心室心尖、大脑顶叶皮层和左肾上极组织行普通病理学及透射电镜检查。结果自主循环恢复后,与假手术组相比,复苏组c Tn I水平显著升高(P0.05);与盐水对照组相比,尼可地尔组c Tn I水平显著降低(P0.05),复苏后心脑肾组织学损伤减轻。结论尼可地尔后适应可减轻猪心脏骤停后心脑肾缺血再灌注损伤。
[Abstract]:Objective to investigate the effect of nicoradil adaptation on ischemia reperfusion injury of heart, brain and kidney in porcine cardiac arrest model. Methods 22 Landrace pigs were randomly divided into two groups: sham operation group (n-4) and resuscitation group (n-18). Cardiopulmonary resuscitation was performed in the resuscitation group after 4 min of electrical stimulation induced ventricular fibrillation. The sham group was given surgery without inducing ventricular fibrillation. Sixteen Landrace pigs in the resuscitation group were successfully resuscitated and randomly divided into two groups: saline control group (NN8) and nicorandil group (NC8). Nikodil group was immediately injected through central vein (150 渭 g/kg) and then intravenously dripped at 3 渭 g / (kg min) to the end of reperfusion, while saline control group was given the same dose of normal saline. The levels of cardiac troponin I (c Tn I) were measured before fibrillation and 5 min,30 min,3 / 6 h after autonomic circulation recovery. The left ventricular apex, the parietal cortex and the upper pole of left kidney were collected at 6 h after the recovery of autonomic circulation. Results the level of c Tn I in the resuscitation group was significantly higher than that in the sham operation group (P0.05), and the c Tn I level in the nicorandil group was significantly lower than that in the saline control group (P0.05). Conclusion adaptation after nicoradil can alleviate ischemia reperfusion injury after cardiac arrest in pigs.
【作者单位】：山东大学附属省立医院急救中心;
【基金】：济南市2012年科学技术发展计划第十九批项目(201219007) 国家自然科学基金(81478133)
【分类号】：R541.78

【相似文献】