定期输血地中海贫血患者血清抗体检测

发布时间：2018-10-11 10:40

【摘要】：目的定期输血患者血清抗体测定和特异性分析。方法采用微柱凝胶抗人球卡方法,对354例长期定期输血的β-地中海贫血患者,进行血清抗体筛查和抗体鉴定。结果 354人中,28人抗体阳性(7.9%),其中13人检出同种抗体(1人无法确定抗体特异性),15人检出自身抗体。共检出特异性同种抗体19个,其中Rh血型抗体15个(78.9%),包括抗-c、抗-E、抗-C和抗-Cw;自身抗体中,1例具有特异性,为抗-C,5例血清与所有鉴定细胞强凝集,均报输血不良反应,其余9人血清与同种细胞弱反应,均未报输血反应。另外,观察到18人次侧配血凝集(5.1%),但血清不与同种细胞反应即主侧配血相合,或因多次输血异体血浆蛋白吸附或药物抗体等原因引起,均未报输血反应。结论长期定期输血的地中海贫血患者血清同种抗体和自身抗体的比率较高,同种抗体以Rh血型抗体为主,应进行Rh Cc Ee相合输血。
[Abstract]:Objective to determine the serum antibody and its specificity in patients with regular blood transfusion. Methods Serum antibody screening and antibody identification were performed in 354 patients with 尾-thalassemia who received blood transfusion for a long time by microcolumn gel anti-human ball card method. Results of 354 cases, 28 were positive (7.9%), of which 13 were homoantibodies (1 could not determine the specificity of antibodies) and 15 were autoantibodies. A total of 19 specific homoantibodies were detected, of which 15 (78.9%) were Rh blood group antibodies, including anti-c, anti-E, anti-C and anti-Cw; autoantibodies. The blood transfusion reaction was not reported in the other 9 cases. In addition, 18 cases of hemagglutination (5.1%) were observed, but the blood did not match with the homologous cells, or was caused by multiple blood transfusions, such as plasma protein adsorption or drug antibody. No blood transfusion reaction was reported. Conclusion the ratio of serum autoantibodies and alloantibodies in thalassemia patients with chronic and regular blood transfusion is higher. Rh blood group antibody is the main antibody and Rh Cc Ee matching transfusion should be carried out.
【作者单位】： 深圳大学第一附属医院深圳市第二人民医院;无锡市第五人民医院;辽宁省血液中心;深圳市血液中心;
【基金】：深圳市科技创新项目(JCYJ20140414170821221) 深圳市第二人民医院院级重点实验室项目资助
【分类号】：R556.61
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本文编号：2263895