结扎兔冠状动脉左前降支血液一氧化氮动态改变研究

发布时间：2018-10-16 20:39

【摘要】：目的：探讨兔急性心肌梗死不同时间,血清一氧化氮浓度的动态变化,及其与肌酸激酶同工酶(CK-MB)、心肌肌钙蛋白T (cTnT)的关系；心肌梗死不同时间后再灌注60分钟时,血清一氧化氮浓度的变化。方法：42只成年健康新西兰大白兔,随机分成为A、B、C、D、E、F组,每组6只。通过结扎兔冠状动脉左前降支,制作兔急性心肌梗死模型,检测不同时间点血清CK-MB、CTnT及NO的浓度。结扎不同时间后,通过松扎左侧冠状动脉前降支60分钟,制作兔心肌缺血再灌注模型,检测松扎后60分钟时血清CK-MB、CTnT及NO的浓度,并全程记录心电图的变化。A组结扎5分钟后松扎60分钟(n=6)、B组结扎10分钟后松扎60分钟(n=6)、C组结扎30分钟后松扎60分钟(n=6)、D组结扎1小时后松扎60分钟(n=6)、E组结扎3小时后松扎60分钟(n=6)、F组结扎6小时后松扎60分钟(n=6),并设假手术组作为对照(n=6)。检测不同时间点血清CK-MB、CTnT及NO的浓度。检测松扎后60分钟时结扎兔冠状动脉前降支5分钟、10分钟、30分钟,1小时、3小时、6小、12小时、24小时和松扎后,记录结扎前后一氧化氮变化及心电图。应用Biovision一氧化氮检测分析试剂盒(比色法),兔肌酸激酶同工酶(CK-MB) ELISA检测试剂盒(上海),兔肌钙蛋白T(CTnT)ELISA检测试剂盒(上海)。用南京建成生物工程研究所提供的CK-MB、CTnT及NO试剂盒,比色法测定各标本血浆中的CK-MB、CTnT及NO的浓度或活力。各项数据均用均数±标准差(i±s)表示。组间比较用单因素方差分析,组内比较用配对T检验。(P0.05)为具有显著性差异。结果：1.CK-MB、CTnT及NO组间比较P0.05,差异有统计学意义。2.各实验组组内比较差异具有统计学意义(P0.05)。3.心电图：结扎后再通的急性心肌梗死兔模型再灌注损伤性心律失常有较高的发生率。与无再灌注损伤性心电图改变组比较,再灌注损伤性心电图改变组血清NO显著降低(P0.01)。结论：1.兔的胸腔结构特殊,给实验操作带来很多方便。兔子大小、价格适中,心腔结构与人类相似,利于实验和效果的评价,适合用于心肌缺血再灌注模型。2.兔AMI模型NO水平显著降低、再灌注不能改善AMI模型的NO降低。3.NO是有可能是早于心电图、血清CK-MB和cTnT诊断AMI的血清标记物。4.血清NO浓度与心肌梗死ST段的抬高与下降之间存在关联,为今后进一步的研究提供依据。
[Abstract]:Objective: to investigate the dynamic changes of serum nitric oxide (no) concentration and its relationship with creatine kinase isoenzyme (CK-MB) and cardiac troponin T (cTnT) in rabbits with acute myocardial infarction (AMI) at different time and 60 minutes after reperfusion. Changes of serum nitric oxide concentration. Methods: 42 healthy adult New Zealand rabbits were randomly divided into two groups (6 in each group). A rabbit model of acute myocardial infarction was made by ligating the left anterior descending branch of coronary artery. Serum CK-MB,CTnT and NO were measured at different time points. After different time of ligation, the left anterior descending coronary artery was ligated for 60 minutes to make the myocardial ischemia-reperfusion model in rabbits. The serum CK-MB,CTnT and NO concentrations were measured at 60 minutes after ligation. Electrocardiogram changes were recorded in the whole course. Group A was ligated for 5 minutes and then loosened for 60 minutes (nong6), B group was ligated for 10 minutes and then loosened for 60 minutes (nong6), C group for 30 minutes after ligation for 30 minutes) (nun6), D group for 1 hour after ligation for 1 hour) (nun6), E group for 3 hours). Posterior ligation was performed 60 minutes after ligation for 6 hours in nong6), F group and sham operation group was used as control group. Serum CK-MB,CTnT and NO concentrations were measured at different time points. The changes of nitric oxide (no) and electrocardiogram (ECG) before and after ligation of anterior descending branch of coronary artery in rabbits were measured 60 minutes after ligation for 5 minutes, 10 minutes, 30 minutes, 1 hour, 3 hours, 6 hours, 12 hours, 24 hours and after ligation. Biovision nitric oxide assay kit (colorimetric method), rabbit creatine kinase isoenzyme (CK-MB) ELISA assay kit (Shanghai) and rabbit troponin T (CTnT) ELISA assay kit (Shanghai) were used. The concentration and activity of CK-MB,CTnT and NO in plasma were determined by colorimetric method with CK-MB,CTnT and NO kit provided by Nanjing Institute of Bioengineering. All the data were expressed as mean 卤standard deviation (I 卤s). Single factor analysis of variance (ANOVA) was used in the comparison between groups, and T test was used in intra-group comparison. (P0.05) there was significant difference. Results: 1. There was significant difference between CK-MBN CTnT and NO group (P 0.05). There were significant differences among the experimental groups (P0.05). Electrocardiogram (ECG): reperfusion injury arrhythmias have a high incidence in rabbit models of acute myocardial infarction after ligation. Compared with the group without reperfusion injury, the serum NO was significantly lower in the reperfusion injury group than in the no reperfusion injury group (P0.01). Conclusion 1. The special structure of the rabbit chest cavity brings a lot of convenience to the experimental operation. The size and price of rabbits are moderate, and the structure of heart cavity is similar to that of human beings. It is suitable for myocardial ischemia-reperfusion model. 2. The level of NO in rabbit AMI model was significantly decreased, but reperfusion could not improve the decrease of NO in AMI model. 3.NO might be the serum marker for diagnosis of AMI in serum CK-MB and cTnT earlier than ECG. 4. There is a correlation between serum NO concentration and elevation and decrease of ST segment in myocardial infarction, which provides basis for further study.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R542.22
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本文编号：2275571