氧化芍药苷对泡沫细胞胆固醇流出的影响

发布时间：2018-11-03 11:46

【摘要】：目的分析氧化芍药苷对THP-1巨噬细胞源性泡沫细胞胆固醇流出的影响。方法 THP-1细胞加入160 nmol/L佛波酯(PMA)24 h,再加入50μg/ml乙酰化低密度脂蛋白(ac-LDL)建立泡沫细胞模型,CCK-8法测定氧化芍药苷的细胞毒性,同位素标记法测定各处理组泡沫细胞胆固醇流出率。共聚焦显微镜观察泡沫细胞内脂肪分化相关蛋白(ADFP)表达变化,Western blot分析泡沫细胞中ADFP、三磷酸腺苷结合盒转运体A1(ABCA1)和过氧化物酶体增殖物激活受体α(PPARα)蛋白表达变化。结果成功建立THP-1巨噬细胞源性泡沫细胞模型。氧化芍药苷毒性分析表明,质量浓度在200.0μg/ml以下无细胞毒性。150.0μg/ml与200.0μg/ml氧化芍药苷处理泡沫细胞,胆固醇流出率分别为(10.317±0.508)%与(11.265±0.713)%,与apo A-1组相比,差异有统计学意义(P0.05),均高于apo A-1组。氧化芍药苷处理的泡沫细胞内ADFP荧光明显减弱。Western blot分析泡沫细胞ADFP表达量降低28%。氧化芍药苷处理组PPARα表达量增加51%,ABCA1表达量增加45%。结论实验表明氧化芍药苷可通过上调PPARα增加ABCA1表达,进而促进泡沫细胞胆固醇流出。
[Abstract]:Objective to analyze the effect of paeoniflorin oxide on cholesterol efflux of THP-1 macrophage derived foam cells. Methods THP-1 cells were treated with 160 nmol/L (PMA) for 24 h, then 50 渭 g/ml acetylated low density lipoprotein (ac-LDL) was added to establish the foam cell model. The cytotoxicity of paeoniflorin was determined by CCK-8 assay. The cholesterol efflux rate of foam cells was measured by isotope labeling method. Observation of (ADFP) expression of adipose differentiation related proteins in foam cells by confocal microscopy, Western blot analysis of ADFP, in foam cells The expression of adenosine triphosphate binding cassette transporter A1 (ABCA1) and peroxisome proliferator-activated receptor 伪 (PPAR 伪) were detected. Results the THP-1 macrophage derived foam cell model was successfully established. The toxicity analysis of paeoniflorin showed that there was no cytotoxicity when the concentration of paeoniflorin was below 200.0 渭 g/ml. 150.0 渭 g/ml and 200.0 渭 g/ml oxidized paeoniflorin were used to treat foam cells. The cholesterol efflux rate was (10.317 卤0.508)% and (11.265 卤0.713)%, respectively, which was significantly higher than that in apo A-1 group (P0.05). The fluorescence of ADFP in foam cells treated with paeoniflorin significantly decreased the expression of ADFP in foam cells analyzed by. Western blot. The expression of PPAR 伪 in paeoniflorin treated group increased by 51% and increased by 45%. Conclusion Paeoniflorin can increase the expression of ABCA1 by up-regulating PPAR 伪, and promote cholesterol efflux of foam cells.
【作者单位】：广东医科大学生物化学与分子生物学教研室;
【分类号】：R543.5

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