生长抑制因子4对内皮细胞增殖、迁移及血管形成相关因子表达的影响

发布时间：2018-11-04 17:34

【摘要】：目的观察生长抑制因子4(ING4)对人脐静脉内皮细胞增殖、迁移及血管内皮生长因子(VEGF)、基质金属蛋白酶2(MMP-2)、基质金属蛋白酶9(MMP-9)活性的影响,探讨ING4对血管内皮细胞及血管生成的调控作用。方法人脐静脉内皮细胞株(HUVECs)体外培养,构建ING4质粒及干扰RNA转染至HUVECs,MTT法检测细胞增殖,Transwell实验检测细胞迁移,实时PCR及Western blotting实验检测血管形成相关因子VEGF、MMP-2、MMP-9 m RNA及蛋白表达水平。结果 MTT及Transwell实验结果显示转染ING4质粒能够抑制HUVECs的增殖及迁移,而转染ING4 si RNA则能够促进HUVECs增殖及迁移,实时PCR及Western blotting实验结果显示转染ING4质粒能够抑制血管形成相关因子VEGF、MMP-2、MMP-9 m RNA及蛋白的表达水平。结论 ING4能够通过抑制血管内皮细胞的增殖及迁移能力,下调血管形成相关因子VEGF、MMP-2、MMP-9的表达,进而抑制新血管形成。
[Abstract]:Objective to investigate the effects of growth inhibitor 4 (ING4) on proliferation, migration and (VEGF), matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) activity of human umbilical vein endothelial cells (HUVECs). To investigate the effect of ING4 on vascular endothelial cells and angiogenesis. Methods Human umbilical vein endothelial cell line (HUVECs) was cultured in vitro. ING4 plasmid was constructed and transfected with interfering RNA to detect cell proliferation. Transwell assay was used to detect cell migration, real-time PCR and Western blotting assay were used to detect angiogenesis related factor VEGF,MMP-2,. MMP-9 m RNA and protein expression level. Results the results of MTT and Transwell experiments showed that the transfection of ING4 plasmid could inhibit the proliferation and migration of HUVECs, while the transfection of ING4 si RNA could promote the proliferation and migration of HUVECs. The results of real-time PCR and Western blotting experiments showed that the transfection of ING4 plasmid could inhibit the angiogenesis related factor VEGF,. The expression level of MMP-2,MMP-9 m RNA and protein. Conclusion ING4 can inhibit angiogenesis by inhibiting the proliferation and migration of vascular endothelial cells and down-regulating the expression of angiogenesis related factor VEGF,MMP-2,MMP-9.
【作者单位】：中国医科大学附属盛京医院病理科;
【分类号】：R54

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