大鼠慢性心肌梗死对前额叶皮质锥体细胞突触活性影响的电生理学研究
发布时间：2018-11-08 07:05
【摘要】：目的:检测大鼠满性心肌梗死所致心绞痛之后,内侧前额叶皮质(medial prefrontal cortex,mPFC)锥体细胞突触传递活性的变化,探讨调节慢性心绞痛可能的中枢机制。方法:雄性成年SD大鼠随机分为假手术组和慢性心肌梗死(chronic myocardial infarction,CMI)手术组,每组5只。手术2周后,制备mPFC区域急性脑片,通过全细胞膜片钳方法记录锥体细胞的兴奋性和抑制性突触电流,比较假手术组和手术组的突触电流活性变化。结果:相较于假手术组动物,手术组动物mPFC内兴奋性突触电流传递明显增强,抑制性突触电流传递明显减弱。同时抑制性中间神经元动作电位释放下降。结论:CMI之后mPFC内锥体细胞的兴奋性突触信号传递增强,可能是由于局部抑制性神经元活性降低所致。鉴于mPFC内锥体细胞活性对于镇痛具有正向效应,这种变化对于mPFC对慢性心绞痛的中枢抑制可能具有积极的意义。
[Abstract]:Aim: to investigate the changes of synaptic transmission activity of pyramidal cells in medial prefrontal cortex (medial prefrontal cortex,mPFC) after angina pectoris induced by full myocardial infarction in rats, and to explore the possible central mechanism of regulating chronic angina pectoris. Methods: male adult SD rats were randomly divided into sham-operated group and chronic myocardial infarction (chronic myocardial infarction,CMI) group with 5 rats in each group. After 2 weeks of operation, acute brain slices of mPFC region were prepared. Excitatory and inhibitory synaptic currents of pyramidal cells were recorded by whole-cell patch clamp method, and the changes of synaptic current activity in sham operation group and operation group were compared. Results: compared with sham operation group, excitatory synaptic current transmission in mPFC was significantly increased and inhibitory synaptic current transmission was significantly decreased in operation group. At the same time, inhibitory action potential release of intermediate neurons decreased. Conclusion: the increase of excitatory synaptic signal transduction in pyramidal cells of mPFC after CMI may be due to the decreased activity of local inhibitory neurons. In view of the positive effect of pyramidal cell activity in mPFC on analgesia, this change may have a positive effect on the central inhibition of chronic angina pectoris by mPFC.
【作者单位】： 西安医学院公共卫生系心理学教研室;
【基金】：陕西省自然科学基础研究计划-青年项目(2017JQ8058) 陕西省教育厅科学研究项目(2017) 陕西省大学生创新创业训练计划平台(201711840024) 西安医学院博士科研启动基金项目(2016DOC10)
【分类号】：R542.22
，
本文编号：2317739
[Abstract]:Aim: to investigate the changes of synaptic transmission activity of pyramidal cells in medial prefrontal cortex (medial prefrontal cortex,mPFC) after angina pectoris induced by full myocardial infarction in rats, and to explore the possible central mechanism of regulating chronic angina pectoris. Methods: male adult SD rats were randomly divided into sham-operated group and chronic myocardial infarction (chronic myocardial infarction,CMI) group with 5 rats in each group. After 2 weeks of operation, acute brain slices of mPFC region were prepared. Excitatory and inhibitory synaptic currents of pyramidal cells were recorded by whole-cell patch clamp method, and the changes of synaptic current activity in sham operation group and operation group were compared. Results: compared with sham operation group, excitatory synaptic current transmission in mPFC was significantly increased and inhibitory synaptic current transmission was significantly decreased in operation group. At the same time, inhibitory action potential release of intermediate neurons decreased. Conclusion: the increase of excitatory synaptic signal transduction in pyramidal cells of mPFC after CMI may be due to the decreased activity of local inhibitory neurons. In view of the positive effect of pyramidal cell activity in mPFC on analgesia, this change may have a positive effect on the central inhibition of chronic angina pectoris by mPFC.
【作者单位】： 西安医学院公共卫生系心理学教研室;
【基金】：陕西省自然科学基础研究计划-青年项目(2017JQ8058) 陕西省教育厅科学研究项目(2017) 陕西省大学生创新创业训练计划平台(201711840024) 西安医学院博士科研启动基金项目(2016DOC10)
【分类号】：R542.22
，
本文编号：2317739
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