宁夏回汉族As患者外周血中ICAM-1基因DNA甲基化与血脂水平的关系

发布时间：2018-11-15 09:27

【摘要】：目的探讨宁夏回、汉族动脉粥样硬化(As)患者外周血中细胞间黏附分子1(ICAM-1)基因DNA启动子区甲基化水平及其与血脂的关系。方法选择经颈部彩色多普勒超声确诊的回、汉族As患者各50例,全自动生化分析仪检测血脂水平,采用实时荧光定量PCR(qRT-PCR)检测外周血ICAM-1 mRNA的表达,巢式降落式甲基化特异性PCR(nt-MSP)检测外周血ICAM-1基因DNA启动子区甲基化程度。结果与回族As组相比,汉族As组甘油三酯显著升高(P0.001);回族As患者ICAM-1表达水平较汉族As组升高(P0.001),ICAM-1 DNA甲基化在回族As患者中下调显著(P0.001);利用相关性分析发现外周血ICAM-1基因甲基化水平与血清中甘油三酯呈负相关(r=-0.5783,P0.001)。结论回族As患者外周血中ICAM-1 DNA低甲基化与As的发生发展相关。
[Abstract]:Objective to investigate the methylation level of intercellular adhesion molecule-1 (ICAM-1) DNA promoter in peripheral blood of patients with atherosclerotic (As) in Hui of Ningxia and Han nationality and its relationship with blood lipids. Methods 50 patients with As in Han nationality and 50 patients with As diagnosed by color Doppler ultrasound were selected. The blood lipid level was detected by automatic biochemical analyzer and the expression of ICAM-1 mRNA in peripheral blood was detected by real-time fluorescence quantitative PCR (qRT-PCR). Nested descent methylation specific PCR (nt-MSP) was used to detect the methylation of DNA promoter of ICAM-1 gene in peripheral blood. Results compared with the Hui As group, the triglyceride level in the As group of the Han nationality was significantly higher than that in the As group of the Hui nationality group (P0.001), and the ICAM-1 expression level in the Hui As group was significantly higher than that in the As group of the Han nationality group (P0.001). Correlation analysis showed that the methylation level of ICAM-1 gene in peripheral blood was negatively correlated with triglyceride in serum (r-0.5783, P0.001). Conclusion hypomethylation of ICAM-1 DNA in peripheral blood of Hui As patients is associated with the occurrence and development of As.
【作者单位】：宁夏医科大学基础医学院;宁夏医科大学总医院烧伤整形外科;
【基金】：宁夏高等学校科学研究项目(NGY2016086)
【分类号】：R543.5

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