MicroRNA-9-3p在2型糖尿病合并不稳定型心绞痛患者血浆中的表达分析
发布时间：2018-11-16 11:18
【摘要】：目的检测microRNA-9-3p(miR-9-3p)在2型糖尿病(T2DM)患者以及T2DM合并不稳定型心绞(T2DM+UA)痛患者血浆中的表达水平,分析miR-9-3p对于T2DM以及T2DM+UA的诊断价值。方法选取2012年1月-2013年6月唐山市工人医院就诊的单纯T2DM患者50例为T2DM组,T2DM+UA患者50例为T2DM+UA组,健康体检人员30例为对照组。抽取患者外周静脉血,提取microRAN(miRNA),应用实时荧光定量聚合酶链反应(q RT-PCR),以miR-423-5p为内参,检测3组血浆中miR-9-3p表达水平,应用受试者工作曲线(ROC)统计分析其对于T2DM以及T2DM+UA的诊断价值。结果 3组血浆miR-9-3p表达水平比较,差异均有统计学意义(P0.05),T2DM组及T2DM+UA组均高于对照组,T2DM+UA组高于T2DM组;miR-9-3p对于T2DM的诊断价值AUC=0.723(95%CI:0.610,0.837,P0.05),miR-9-3p对于T2DM+UA的诊断价值AUC=0.972(95%CI:0.963,0.981,P0.05)。结论 miR-9-3p在T2DM以及T2DM+UA患者血浆表达水平升高,miR-9-3p有望作为T2DM以及T2DM+UA早期诊断的分子标志物。
[Abstract]:Objective to detect the expression of microRNA-9-3p (miR-9-3p) in plasma of patients with type 2 diabetes mellitus (T2DM) and patients with T2DM complicated with unstable cardiac strangulation (T2DM UA), and to analyze the diagnostic value of miR-9-3p for T2DM and T2DM UA. Methods from January 2012 to June 2013, 50 patients with T2DM were selected as T2DM group, 50 patients with T2DM UA as T2DM UA group and 30 healthy persons as control group. The peripheral venous blood was extracted and microRAN (miRNA), was extracted by real-time fluorescence quantitative polymerase chain reaction (Q RT-PCR). MiR-423-5p was used as internal reference to detect the expression of miR-9-3p in plasma of three groups. The diagnostic value of (ROC) for T2DM and T2DM UA was analyzed. Results there were significant differences in plasma miR-9-3p expression among the three groups (P0.05). The expression of miR-9-3p in T2DM group and T2DM UA group was higher than that in control group, and that in T2DM UA group was higher than that in T2DM group. The diagnostic value of miR-9-3p for T2DM (95 CI: 0.610 / 0.837) and miR-9-3p for T2DM UA (95 CI: 0.963 / 0.981p0.05). Conclusion the expression of miR-9-3p in plasma of patients with T2DM and T2DM UA is increased. MiR-9-3p may be a molecular marker for early diagnosis of T2DM and T2DM UA.
【作者单位】： 河北省唐山市工人医院内分泌二科;河北省唐山市工人医院心内四科;
【分类号】：R541.4;R587.1
[Abstract]:Objective to detect the expression of microRNA-9-3p (miR-9-3p) in plasma of patients with type 2 diabetes mellitus (T2DM) and patients with T2DM complicated with unstable cardiac strangulation (T2DM UA), and to analyze the diagnostic value of miR-9-3p for T2DM and T2DM UA. Methods from January 2012 to June 2013, 50 patients with T2DM were selected as T2DM group, 50 patients with T2DM UA as T2DM UA group and 30 healthy persons as control group. The peripheral venous blood was extracted and microRAN (miRNA), was extracted by real-time fluorescence quantitative polymerase chain reaction (Q RT-PCR). MiR-423-5p was used as internal reference to detect the expression of miR-9-3p in plasma of three groups. The diagnostic value of (ROC) for T2DM and T2DM UA was analyzed. Results there were significant differences in plasma miR-9-3p expression among the three groups (P0.05). The expression of miR-9-3p in T2DM group and T2DM UA group was higher than that in control group, and that in T2DM UA group was higher than that in T2DM group. The diagnostic value of miR-9-3p for T2DM (95 CI: 0.610 / 0.837) and miR-9-3p for T2DM UA (95 CI: 0.963 / 0.981p0.05). Conclusion the expression of miR-9-3p in plasma of patients with T2DM and T2DM UA is increased. MiR-9-3p may be a molecular marker for early diagnosis of T2DM and T2DM UA.
【作者单位】： 河北省唐山市工人医院内分泌二科;河北省唐山市工人医院心内四科;
【分类号】：R541.4;R587.1
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