二脒那秦在肺动脉高压模型大鼠中的预防和治疗作用

发布时间：2018-11-18 17:24

【摘要】：目的探究二脒那秦(DIZE)对肺动脉高压(PAH)模型大鼠的预防及治疗作用。方法采用左肺叶切除联合野百合碱(MCT)腹壁皮下注射建立PAH大鼠模型,100只成年雄性Wistar大鼠随机分成空白对照组(A组)、DIZE对照组(B组)、PAH模型组(C组)、DIZE处理PAH模型组(D组)和(DIZE+C-16)处理PAH模型组(E组),利用活性荧光共振能量转移(FRET)和酶联免疫吸附试验(ELISA)法测定血管紧张素转化酶2(ACE2)、血清IL-6及IL-8水平,并测定平均肺动脉压(m PAP)、右心室肥厚指数(RVHI),计算中膜厚度与肺动脉外径的比例(WT)、内膜增生评分,并通过弹力纤维染色分析肺血管病变。结果 RVHI、ACE2酶活性、WT及内膜增生评分比较:与A组比较,C组、D组和E组差异有统计学意义(P0.05);D组和E组差异有统计学意义(P0.05)。五个组在各个指标整体分析中差异有统计学意义(P0.05)。结论二脒那秦可以提高ACE2酶活性,并有效降低m PAP、RVHI和WT,同时减轻肺动脉中膜肥厚,并抑制肺小动脉内膜增生。该研究结论为二脒那秦治疗人类PAH提供实验基础。
[Abstract]:Objective to investigate the preventive and therapeutic effects of dimidine (DIZE) on pulmonary hypertension (PAH) rats. Methods PAH rat model was established by left lobectomy and subcutaneous injection of monocrotaline (MCT) into abdominal wall. 100 adult male Wistar rats were randomly divided into control group (group A,), DIZE control group) and group B (), PAH model group, group C). PAH model group (D group) and PAH model group (DIZE C-16) were treated with DIZE. Angiotensin converting enzyme 2 (ACE2) was measured by active fluorescence resonance energy transfer (FRET) and enzyme-linked immunosorbent assay (ELISA). The levels of serum IL-6 and IL-8 were measured, and the mean pulmonary artery pressure (m PAP),) right ventricular hypertrophy index (RVHI),) was measured to calculate the ratio of intimal hyperplasia score (WT),) to the ratio of medial thickness to pulmonary artery diameter. Pulmonary vascular lesions were analyzed by elastic fiber staining. Results the activity of RVHI,ACE2, WT and intimal hyperplasia score: compared with group A, group C, group D and group E had significant differences (P0.05) between); D group and E group (P0.05). There were significant differences among the five groups in the whole analysis of each index (P0.05). Conclusion Diamidinazin can increase the activity of ACE2, decrease m PAP,RVHI and WT, reduce pulmonary artery intimal hypertrophy and inhibit intimal hyperplasia of pulmonary arterioles. The results provide experimental basis for the treatment of human PAH with dimidine.
【作者单位】：石河子大学医学院第一附属医院心内科;
【分类号】：R544.1;R-332

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