二氧化硫及其衍生物对大鼠心肌细胞胶原蛋白表达的影响
发布时间：2018-11-20 21:40
【摘要】：以100μmol·L~(-1)亚硫酸氢钠对H9C2心肌细胞染毒不同时间(3,6,12,24 h),采用Wistar大鼠作为模型进行整体动物染毒,SO_2组动式吸入SO_2(7 mg·m~(-3))28 d,每天4 h;SO~(2+)NALC(N-乙酰半胱氨酸)组吸入同样条件的SO_2,且自SO_2染毒之日起隔天腹腔注射50 mg·kg-1(b.w.)NALC,对照组吸入新鲜空气并注射生理盐水.测定大鼠心脏组织和H9C2细胞内ROS含量;采用荧光定量PCR和Western blot分析I型胶原(Col1a1)和III型胶原(Col3a1)的mRNA转录和蛋白表达水平.结果显示SO_2及其衍生物引起的氧化应激显著增加了活性氧(ROS)的产生;SO_2及其衍生物不能诱导大鼠心脏组织和H9C2细胞中Col1a1和Col3a1 mRNA转录水平的显著改变,但Col1a1和Col3a1的蛋白表达水平显著升高;同时NALC可减少心脏组织中ROS的产生,有效抑制SO_2吸入后Col1a1和Col3a1蛋白表达的上升.提示SO_2吸入后可能通过产生ROS最终导致胶原蛋白表达的增加.
[Abstract]:H9C2 cardiomyocytes were exposed to 100 渭 mol L ~ (-1) bisulfite sodium at different time (3 ~ 6 ~ 12 ~ (-1) h),). SO_2 group was exposed to SO_2 (7 mg m ~ (-3) for 28 days by dynamic inhalation of SO_2 (7 mg m ~ (-3). 4 hours per day; SO~ (2) NALC (N-acetylcysteine) group inhaled the same SO_2, and injected 50 mg kg-1 (B. w.) NALC, control group with fresh air and normal saline every other day from the day of SO_2 exposure. The levels of mRNA transcription and protein expression of type I collagen (Col1a1) and III type collagen (Col3a1) were determined by fluorescence quantitative PCR and Western blot. The results showed that oxidative stress induced by SO_2 and its derivatives significantly increased the production of reactive oxygen (ROS). SO_2 and its derivatives could not induce significant changes of Col1a1 and Col3a1 mRNA transcription levels in rat heart tissues and H9C2 cells, but the expression levels of Col1a1 and Col3a1 were significantly increased. At the same time, NALC can reduce the production of ROS in heart tissue and inhibit the increase of Col1a1 and Col3a1 protein expression after SO_2 inhalation. These results suggest that SO_2 inhalation may result in an increase in collagen expression through the production of ROS.
【作者单位】： 山西大学环境与资源学院;广州市环境暴露与健康重点实验室暨南大学环境学院;
【基金】：国家自然科学基金(No.21007036) 广州市环境暴露与健康重点实验室开放基金(No.GZKLEEH201612) 环境化学与生态毒理学国家重点实验室开放基金资助项目(No.KF2016-17)~~
【分类号】：X51;R54
本文编号：2346120
[Abstract]:H9C2 cardiomyocytes were exposed to 100 渭 mol L ~ (-1) bisulfite sodium at different time (3 ~ 6 ~ 12 ~ (-1) h),). SO_2 group was exposed to SO_2 (7 mg m ~ (-3) for 28 days by dynamic inhalation of SO_2 (7 mg m ~ (-3). 4 hours per day; SO~ (2) NALC (N-acetylcysteine) group inhaled the same SO_2, and injected 50 mg kg-1 (B. w.) NALC, control group with fresh air and normal saline every other day from the day of SO_2 exposure. The levels of mRNA transcription and protein expression of type I collagen (Col1a1) and III type collagen (Col3a1) were determined by fluorescence quantitative PCR and Western blot. The results showed that oxidative stress induced by SO_2 and its derivatives significantly increased the production of reactive oxygen (ROS). SO_2 and its derivatives could not induce significant changes of Col1a1 and Col3a1 mRNA transcription levels in rat heart tissues and H9C2 cells, but the expression levels of Col1a1 and Col3a1 were significantly increased. At the same time, NALC can reduce the production of ROS in heart tissue and inhibit the increase of Col1a1 and Col3a1 protein expression after SO_2 inhalation. These results suggest that SO_2 inhalation may result in an increase in collagen expression through the production of ROS.
【作者单位】： 山西大学环境与资源学院;广州市环境暴露与健康重点实验室暨南大学环境学院;
【基金】：国家自然科学基金(No.21007036) 广州市环境暴露与健康重点实验室开放基金(No.GZKLEEH201612) 环境化学与生态毒理学国家重点实验室开放基金资助项目(No.KF2016-17)~~
【分类号】：X51;R54
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