血清miR-663、miR-126和miR-370对冠心病患者支架内再狭窄的预测价值

发布时间：2018-11-25 17:44

【摘要】：目的研究血清微小RNA(miRNA)中的miR-663、miR-126和miR-370在冠心病患者支架植入术后的表达情况,分析以上3种miRNA对支架内再狭窄(ISR)的预测价值。方法研究纳入175例冠心病支架植入术后并随访复查冠状动脉造影的患者,根据造影结果分为ISR组(n=66)和无ISR组(n=109)。分析比较两组患者的临床资料。收集所有患者的血清,通过实时荧光定量PCR检测血清miR-663、miR-126和miR-370的表达情况。应用统计学方法分析以上3个miRNA预测ISR的价值。结果 175例支架植入术后冠心病患者中发生ISR的共66例。ISR组吸烟率更高(P0.05);ISR组血糖、低密度脂蛋白胆固醇、高敏C反应蛋白(hs-CRP)水平均高于无ISR组(P0.01)。ISR组血清miR-663、miR-126表达量低于无ISR组,而miR-370表达量高于无ISR组(均P0.01)。Logistic回归分析表明,miR-663、miR-126表达水平与ISR发生相关,吸烟、hs-CRP是ISR的独立危险因素。受试者工作特征曲线结果表明,miR-663、miR-126和miR-370预测ISR的曲线下面积(AUC)分别为0.772、0.720和0.650;而miR-663联合miR-126预测ISR的AUC为0.909,优于单一miRNA的预测效能。结论冠心病支架植入术后ISR患者血清miR-663、miR-126表达下降,miR-370表达增加。miR-663、miR-126和miR-370可用于预测ISR的发生,miR-663联合miR-126可明显提高预测ISR的效能。
[Abstract]:Objective to study the expression of miR-663,miR-126 and miR-370 in serum microRNA (miRNA) after stent implantation in patients with coronary heart disease (CHD), and to analyze the predictive value of these three miRNA for restenosis (ISR) in stent. Methods 175 patients with coronary artery disease underwent stent implantation and were followed up for coronary angiography. According to the results of coronary angiography, they were divided into two groups: ISR group (n = 66) and no ISR group (n = 109). The clinical data of the two groups were analyzed and compared. The expression of miR-663,miR-126 and miR-370 was detected by real-time fluorescence quantitative PCR. The value of the above three miRNA in predicting ISR was analyzed by statistical method. Results there were 66 cases of ISR in 175 patients with coronary heart disease after stent implantation. The smoking rate of ISR group was higher than that of ISR group (P0.05). The levels of blood glucose, low density lipoprotein cholesterol and Gao Min C-reactive protein (hs-CRP) in ISR group were significantly higher than those in no ISR group (P0.01). ISR group). However, the expression of miR-370 was higher than that without ISR (all P0.01). Logistic regression analysis showed that the level of miR-663,miR-126 expression was associated with ISR, smoking and hs-CRP were independent risk factors of ISR. The results of operating characteristic curve showed that the area under the curve predicted by miR-663,miR-126 and miR-370 was 0.7720.720 and 0.650, respectively. The AUC of ISR predicted by miR-663 combined with miR-126 was 0.909, which was superior to that of single miRNA. Conclusion the expression of serum miR-663,miR-126 decreased and the expression of miR-370 increased after stent implantation in patients with coronary heart disease. MiR-663,miR-126 and miR-370 can be used to predict the occurrence of ISR. MiR-663 combined with miR-126 can significantly improve the effectiveness of ISR prediction.
【作者单位】：武汉亚洲心脏病医院心内科;
【基金】：武汉市卫计委课题(WX14C78)
【分类号】：R541.4

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