间隙性连接蛋白37对动脉粥样硬化中单核巨噬细胞电流密度的影响
发布时间：2018-11-28 07:19
【摘要】：目的国内外关于间隙性连接蛋白37(Cx37)基因改变巨噬细胞内外的电流密度,使得动脉粥样硬化进一步发展的研究甚少。文中旨在观察Cx37对动脉粥样硬化中巨噬细胞电流密度的影响。方法选取30只Wistar大鼠,Cx37+(保留Cx37基因)、Cx37-(沉默Cx37基因)各15只。采用高脂饮食建立动脉粥样硬化模型;根据标本采集部位分为4组:Cx37+斑块组、Cx37-斑块组(取自斑块)及Cx37+血液组、Cx37-血液组(取自血液),用RT-PCR检测Cx37+大鼠不同部位Cx37的表达;分离Cx37+、Cx37-大鼠血液中及斑块处单核巨噬细胞并培养,用全细胞记录法测定电流密度并进行比较。结果 Cx37+大鼠斑块中单核巨噬细胞中Cx37的相对表达水平显著高于血液[(1.1±0.02)vs(0.6±0.03)],差异有统计学意义(P=0.010)。Cx37+、Cx37-大鼠均行能谱CT检测鼠颈动脉斑块检测,验证了动物模型的成功建立。80、120、160 ms时,Cx37+斑块组电流密度[(0.61±0.06)、(0.67±0.07)、(0.91±0.03)A/cm~2]显著高于Cx37+血液组[(0.49±0.02)、(0.61±0.03)、(0.67±0.02)A/cm~2]、Cx37-斑块组[(0.48±0.02)、(0.60±0.02)、(0.64±0.02)A/cm~2]、Cx37-血液组[(0.49±0.02)、(0.59±0.02)、(0.64±0.02)A/cm~2];200、240、320 ms时亦显著高于Cx37+血液组、Cx37-斑块组、Cx37-血液组(P0.05)。结论 Cx37对动脉粥样硬化中单核巨噬细胞斑块处电流密度的影响比外周血液更为显著,可促使巨噬细胞继续活化,动脉粥样硬化进一步发展。
[Abstract]:Objective to investigate the effect of gap junction protein 37 (Cx37) gene on the current density of macrophages and make the further development of atherosclerosis rare. The aim of this study was to observe the effect of Cx37 on the current density of macrophages in atherosclerosis. Methods 30 Wistar rats with Cx37 (Cx37 gene) and Cx37- (silent Cx37 gene) were selected. Atherosclerosis model was established by high-fat diet. The samples were divided into four groups: Cx37 plaque group, Cx37- plaque group (taken from plaque) and Cx37 blood group, Cx37- blood group (taken from blood). The expression of Cx37 in different parts of Cx37 rats was detected by RT-PCR. Mononuclear macrophages were isolated from the blood and plaque of Cx37 and Cx37- rats and cultured. The current density was measured by whole-cell recording method and compared. Results the relative expression of Cx37 in mononuclear macrophages in plaque of Cx37 rats was significantly higher than that in blood [(1.1 卤0.02) vs (0.6 卤0.03)], and the difference was statistically significant (P0. 010). Cx37. Cx37- rats were detected carotid plaque by energy dispersive CT. The results showed that the current density of Cx37 plaque group was (0.61 卤0.06), (0.67 卤0.07) at 80120160 ms. (0. 91 卤0. 03) A/cm~2 was significantly higher than that in Cx37 blood group [(0. 49 卤0. 02), () 0. 61 卤0. 03), (0. 67 卤0. 02 A/cm~2], Cx37- plaque group [(0. 48 卤0. 02), () 0. 60 卤0. 02]. (0. 64 卤0. 02 A/cm~2), Cx37- blood group [(0. 49 卤0. 02), () 0. 59 卤0. 02), (0. 64 卤0. 02 A/cm~2]; At 200240320 ms, it was also significantly higher than that in Cx37 blood group, Cx37- plaque group and Cx37- blood group (P0.05). Conclusion the effect of Cx37 on the current density in plaque of mononuclear macrophages in atherosclerosis is more significant than that in peripheral blood, which can promote the activation of macrophages and the further development of atherosclerosis.
【作者单位】： 云南大学博士后流动站;无锡市人民医院心脏中心;昆明医科大学第一附属医院心内科;
【分类号】：R543.5
本文编号：2362198
[Abstract]:Objective to investigate the effect of gap junction protein 37 (Cx37) gene on the current density of macrophages and make the further development of atherosclerosis rare. The aim of this study was to observe the effect of Cx37 on the current density of macrophages in atherosclerosis. Methods 30 Wistar rats with Cx37 (Cx37 gene) and Cx37- (silent Cx37 gene) were selected. Atherosclerosis model was established by high-fat diet. The samples were divided into four groups: Cx37 plaque group, Cx37- plaque group (taken from plaque) and Cx37 blood group, Cx37- blood group (taken from blood). The expression of Cx37 in different parts of Cx37 rats was detected by RT-PCR. Mononuclear macrophages were isolated from the blood and plaque of Cx37 and Cx37- rats and cultured. The current density was measured by whole-cell recording method and compared. Results the relative expression of Cx37 in mononuclear macrophages in plaque of Cx37 rats was significantly higher than that in blood [(1.1 卤0.02) vs (0.6 卤0.03)], and the difference was statistically significant (P0. 010). Cx37. Cx37- rats were detected carotid plaque by energy dispersive CT. The results showed that the current density of Cx37 plaque group was (0.61 卤0.06), (0.67 卤0.07) at 80120160 ms. (0. 91 卤0. 03) A/cm~2 was significantly higher than that in Cx37 blood group [(0. 49 卤0. 02), () 0. 61 卤0. 03), (0. 67 卤0. 02 A/cm~2], Cx37- plaque group [(0. 48 卤0. 02), () 0. 60 卤0. 02]. (0. 64 卤0. 02 A/cm~2), Cx37- blood group [(0. 49 卤0. 02), () 0. 59 卤0. 02), (0. 64 卤0. 02 A/cm~2]; At 200240320 ms, it was also significantly higher than that in Cx37 blood group, Cx37- plaque group and Cx37- blood group (P0.05). Conclusion the effect of Cx37 on the current density in plaque of mononuclear macrophages in atherosclerosis is more significant than that in peripheral blood, which can promote the activation of macrophages and the further development of atherosclerosis.
【作者单位】： 云南大学博士后流动站;无锡市人民医院心脏中心;昆明医科大学第一附属医院心内科;
【分类号】：R543.5
【相似文献】
相关期刊论文 前7条
1 奚永志;叶根耀;蒋本荣;;单核巨噬细胞及前列腺素E_2在正常人粒系造血调控中的作用[J];临床血液学杂志;1991年03期
2 包兴才,李同义;再障病人外周单核巨噬细胞某些造血调控功能异常[J];国外医学.输血及血液学分册;1992年06期
3 浦部晶夫 ,李艳;白血病、恶性淋巴瘤的治疗与集落刺激因子[J];日本医学介绍;1990年11期
4 舒茂琴,何作云,周建新,王国超,方玉强;单核巨噬细胞摄取极低密度脂蛋白机制的初步研究[J];中华老年医学杂志;2001年06期
5 何志藩;胸腹膜渗出液内的单核巨噬细胞与间皮细胞的鉴别[J];国外医学参考资料(肿瘤学分册);1976年01期
6 舒茂琴,何作云,周建新,王国超,方玉强;VLDL和ox-LDL对单核巨噬细胞的作用及机制探讨[J];临床心血管病杂志;2002年11期
7 张嵩阳;;单核巨噬细胞α_M β2整合素抑制15-LO诱导的CD36表达[J];生理科学进展;2012年01期
，本文编号：2362198
本文链接：https://www.wllwen.com/yixuelunwen/xxg/2362198.html
最近更新
教材专著
