重组人血小板生成素诱导小鼠反应性血小板增多症的病理变化

发布时间：2018-11-28 21:07

【摘要】：目的:为了研究重组人血小板生成素(rhTPO)诱导小鼠反应性血小板增多症的病理变化。方法:将48只4周龄SPF级BALB/c小鼠,随机分为rhTPO组和对照组,各24只。rhTPO组每只小鼠每日腹腔注射rhTPO 500μg/(kg·d),0.3 ml/只,对照组每只小鼠每日腹腔注射生理盐水0.3 m L/只,连续注射10 d。实验第3、5、7和10天,取2组小鼠各6只拉颈处死,检测两组小鼠的外周血细胞数量、血小板(platelet,PLT)聚集实验(platelet aggregation test,PAgT)、血小板ATP释放试验、血浆D-二聚体(D-dimer)浓度测定、小鼠血栓表现的观察、骨髓活检、脾、肝系数及脾、肝病理检查。结果:rhTPO组PLT数量在第3天明显高于对照组(P0.05),在第5、7和10天明显高于对照组(P0.01)。rhTPO组5 min内最大凝集率和血小板ATP释放量在3、5、7和10天与对照组均无统计学差异(P0.05)。rhTPO组血浆D-dimer浓度在第3、5、7和10天明显逐渐升高(P0.01),在第3天明显高于对照组(P0.05),在第5、7和10天明显高于对照组(P0.01)。2组小鼠均无血栓表现。rhTPO组在第3、5、7和10天巨核细胞大量增生,体积增大,且随时间日益明显。rhTPO组脾系数在第5、7天均明显高于对照组(P0.05),在第10 d明显高于对照组(P0.01),rhTPO组肝系数在第10天明显高于对照组(P0.05)。rhTPO组脾脏巨核系和脾体积在第3天内不变,但脾脏巨核系在第5、7和10天逐渐增生,第10天尚可见红系增生,脾体积在第5、7和10天逐渐增大。肝脏在第10天可见红系、粒系和巨核系增生且体积增大。结论:rhTPO诱导小鼠反应性血小板增多症的病理变化:骨髓中巨核细胞大量增生,外周血PLT数量急剧升高,但生成的PLT功能正常,初期,脾、肝未发生病理变化,中后期可刺激脾、肝先后髓外造血,体积先后增大。中后期可刺激脾脏巨核系逐渐增生,后期尚可见红系增生,脾体积也逐渐增大。肝在后期且可见红系、粒系和巨核系三系增生且体积增大。
[Abstract]:Aim: to study the pathological changes of reactive thrombocytosis induced by recombinant human thrombopoietin (rhTPO) in mice. Methods: Forty-eight 4-week-old SPF BALB/c mice were randomly divided into two groups: rhTPO group and control group, 24 mice in the rhTPO group were intraperitoneally injected with rhTPO 500 渭 g / (kg d), 0.3 ml/ daily. Each mouse in the control group was intraperitoneally injected with normal saline 0.3 mL / day for 10 days. On the 7th and 10th day of the experiment, 6 mice in each group were killed by neck pulling. The number of peripheral blood cells (PBMC), platelet aggregation test (platelet aggregation test,PAgT) and platelet ATP release test were detected in both groups. Plasma concentration of D-dimer (D-dimer), observation of thrombosis in mice, bone marrow biopsy, spleen, liver coefficient and spleen, liver pathological examination. Results: the number of PLT in the rhTPO group was significantly higher than that in the control group on the 3rd day (P0.05), and on the 7th and 10th day in the rhTPO group, it was significantly higher than that in the control group (P0.01). RhTPO group). On the 7th and 10th day, there was no significant difference between the control group and the control group (P0.05). The plasma D-dimer concentration in the). RhTPO group increased gradually on the 7th and 10th day (P0.01), and on the third day, it was significantly higher than that in the control group (P0.05). On the 7th and 10th day, the number of megakaryocytes in the rhTPO group was significantly higher than that in the control group (P0.01). The spleen coefficient of rhTPO group was significantly higher than that of control group on the 7th day (P0.05), and higher than that of control group (P0.01) on the 10th day. The liver coefficient of the rhTPO group was significantly higher than that of the control group on the 10th day (P0.05) the splenic megakaryocyte system and splenic volume in the). RhTPO group did not change at the 3rd day, but the splenic megakaryocytic system gradually proliferated on the 7th and 10th day, and the erythroid proliferation was observed on the 10th day. The volume of spleen gradually increased on the 7th and 10th day. Erythroid, granulocyte and megakaryocyte proliferated and enlarged in the liver on day 10. Conclusion: the pathological changes of reactive thrombocytopenia induced by rhTPO: the megakaryocyte proliferation in bone marrow and the number of PLT in peripheral blood increased sharply, but the function of PLT was normal, and no pathological changes occurred in spleen and liver at the initial stage. The middle and late stage can stimulate spleen, liver successively extramedullary hematopoiesis, volume successively increase. The splenic megakaryocyte was gradually proliferated in the middle and late stage, and the erythroid proliferation was seen in the later stage, and the splenic volume increased gradually. The erythroid, granulocyte and megakaryocyte were proliferated and enlarged in the later stage of liver.
【作者单位】：湖北理工学院医学院;黄石市中心医院血液内科;
【基金】：湖北省教育厅科技研究计划指导项目(编号:B2014027)
【分类号】：R558.3

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