缺氧诱导因子1α及血管内皮生长因子在缺氧性肺动脉高压新生大鼠肺组织的表达研究

发布时间：2018-12-30 21:35

【摘要】：目的:通过研究缺氧诱导因子-1α(hypoxia-inducible factor-1 alpha,HIF-1α)及血管内皮生长因子(vascular endothelial growth factor,VEGF)在缺氧性肺动脉高压(hypoxia-induced plmonary hypertension,HPH)新生大鼠肺组织中的mRNA和蛋白质表达以及与平均肺动脉压力(mean pulmonary artery pressure,m PAP)的关系,探讨HIF-1α和VEGF在HPH发病机制中的作用。方法:把80只Wistar新生鼠根据随机数字表法划分成HPH组和空白组(C组),分别在造模3、7、14、21天时取HPH组和实验组各10只大鼠,通过检测mPAP判断造模是否成功;处死大鼠后测定HIF-1α及VEGF mRNA在肺组织的表达水平,利用Western blot方法测定HIF-1α及VEGF的蛋白表达进行分析。结果:1.造模3、7、14、21天时,HPH组mPAP较空白组显著增高,差异有统计学意义(P0.05);2.造模3、7、14天时HPH组肺组织HIF-1α的mRNA表达较空白组升高,差异有统计学意义(P0.05),造模7、14、21天时,HPH组VEGF mRNA表达水平均明显高于空白组(P0.05)。3.造模7天时HPH组肺组织HIF-1α蛋白表达显著比空白组增多(P0.05);缺氧7、14、21天时,HPH组VEGF蛋白表达均显著比空白组增多(P0.05)。相关分析研究显示,HPH组在造模第3、7、14、21天时HIF-1α的蛋白表达与mPAP变化大致成正相关(P0.05),造模第7、14、21天,VEGF蛋白质表达与mPAP变化大致成正相关。结论VEGF作为HIF-lα的下游调控因子在新生大鼠HPH疾病发生发展过程里起到重要作用。
[Abstract]:Objective: to investigate the role of hypoxia inducible factor-1 伪 (hypoxia-inducible factor-1 alpha,HIF-1 伪) and vascular endothelial growth factor (vascular endothelial growth factor,VEGF) in hypoxic pulmonary hypertension (hypoxia-induced plmonary hypertension,). To explore the role of HIF-1 伪 and VEGF in the pathogenesis of HPH. Methods: 80 neonatal Wistar rats were randomly divided into HPH group and blank group (C group). 10 rats in HPH group and 10 rats in experimental group were taken from HPH group and experimental group on day 21 of model making. MPAP was detected to determine whether the model was successful or not. The expression of HIF-1 伪 and VEGF mRNA in lung tissue was determined after the rats were killed, and the protein expression of HIF-1 伪 and VEGF was analyzed by Western blot method. Results: 1. The mPAP of HPH group was significantly higher than that of blank group (P0.05). The mRNA expression of HIF-1 伪 in lung tissue of HPH group was significantly higher than that of blank group on day 14 (P0.05), and the expression level of VEGF mRNA in HPH group was significantly higher than that in control group (P0.05) at day 21 (P0.05). The expression of HIF-1 伪 protein in lung tissue of HPH group was significantly higher than that of blank group at 7 days (P0.05), and the expression of VEGF protein in HPH group was significantly higher than that in blank group at day 1421 of hypoxia (P0.05). Correlation analysis showed that the protein expression of HIF-1 伪 was positively correlated with the changes of mPAP in the HPH group on the 3rd day (P0.05), and the expression of VEGF protein was positively correlated with the change of mPAP on the 21st day (P0.05). Conclusion VEGF, as the downstream regulatory factor of HIF-l 伪, plays an important role in the pathogenesis and development of HPH in neonatal rats.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R544.1

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