胰岛素抵抗及脂联素缺乏对小鼠心肌重构的影响

发布时间：2019-01-07 20:07

【摘要】：目的 :研究胰岛素抵抗(IR)及脂联素(APN)缺乏对小鼠心肌重构的影响。方法 :选择16只野生C57小鼠随机分为对照组和IR组,每组8只;16只APN基因敲除(APNKO)C57小鼠随机分为APNKO组和APNKO+IR组,每组各8只。对照组和APNKO组给予普通饲料,IR组和APNKO+IR组给予高脂饲料诱导产生IR。喂养12周后取血测定总胆固醇、甘油三酯、空腹血糖和空腹胰岛素水平,取心脏测量心脏重量和左心室重量。取左心室心肌做苏木素伊红(HE)染色和马松(Masson)染色,观察各组心肌结构改变及纤维化的程度;用免疫组化法和蛋白免疫印迹法检测心肌中基质金属蛋白酶-9(MMP-9)和APN表达量的差异。结果 :与对照组比较,IR组、APNKO组和APNKO+IR组中小鼠的总胆固醇、甘油三酯、空腹血糖和空腹胰岛素、心脏重量和左心室重量均有增加(P0.05);HE染色及Masson染色结果显示:与对照组比较,IR组、APNKO组和APNKO+IR组中小鼠的心肌肥大程度更高;免疫组化及蛋白免疫印迹结果显示:与对照组相比,IR组、APNKO组和APNKO+IR组中小鼠心肌APN表达明显减低,心肌MMP-9表达明显增加(P0.05)。结论 :APN缺乏和IR导致心肌重构,且两者起协同促进作用。
[Abstract]:Aim: to study the effects of insulin resistant (IR) and adiponectin (APN) deficiency on myocardial remodeling in mice. Methods: sixteen wild C57 mice were randomly divided into control group and IR group, and 16 APN gene knockout (APNKO) C57 mice were randomly divided into APNKO group and APNKO IR group with 8 mice in each group. The control group and APNKO group were given ordinary diet, and the IR group and APNKO IR group were given high fat diet to induce IR. production. After 12 weeks of feeding, total cholesterol, triglyceride, fasting blood glucose and fasting insulin were measured. Heart weight and left ventricular weight were measured. Left ventricular myocardium was stained with hematoxylin eosin (HE) and Ma Song (Masson) to observe the changes of myocardial structure and the degree of fibrosis. The expression of matrix metalloproteinase-9 (MMP-9) and matrix metalloproteinase-9 (APN) in myocardium were detected by immunohistochemistry and Western blot. Results: compared with the control group, the total cholesterol, triglyceride, fasting blood glucose, fasting insulin, heart weight and left ventricular weight were increased in IR, APNKO and APNKO IR groups (P0.05). The results of HE staining and Masson staining showed that the degree of myocardial hypertrophy in IR group, APNKO group and APNKO IR group was higher than that in control group. The results of immunohistochemistry and Western blot showed that compared with the control group, the expression of myocardial APN in IR group, APNKO group and APNKO IR group was significantly decreased, and the expression of myocardial MMP-9 was significantly increased (P0.05). Conclusion: APN deficiency and IR lead to myocardial remodeling, and they play a synergistic role in promoting myocardial remodeling.
【作者单位】：宁夏医科大学心血管内科;宁夏医科大学总医院心脏中心干部病房;宁夏医科大学基础医学院;
【基金】：国家自然科学基金资助项目(81360026)
【分类号】：R54

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