压力超负荷大鼠心肌胶原代谢的动态变化

发布时间：2019-02-08 21:05

【摘要】：目的:探讨压力超负荷大鼠心肌胶原代谢的动态变化。方法:采用腹主动脉缩窄法制备压力超负荷大鼠模型,并以假手术组为对照,分别于术后第3周、4周、8周和12周取材,称量大鼠体质量、心脏质量和左心室质量,计算心脏质量指数(heart mass index,HMI)和左心室质量指数(left ventricle mass index,LVMI);心肌组织Masson染色观察心肌胶原纤维沉积情况;样本碱水解法检测心肌组织羟脯氨酸(hydroxyproline,HYP)含量;ELISA法检测血清Ⅰ型前胶原羧基端肽(procollagen typeⅠcarboxy-terminal peptide,PⅠCP)、Ⅲ型前胶原氨基端肽(procollagen typeⅢamino-terminal peptide,PⅢNT)和Ⅰ型胶原C端肽(collagen C telopeptide typeⅠ,CTX-Ⅰ)水平。结果:与假手术组相比,模型组大鼠心肌血管周围及间质可见胶原纤维沉积,心肌胶原容积分数明显增高(P0.01),且随时间延长胶原沉积明显增多;模型组大鼠HMI、LVMI和HYP均明显增加(P0.05),且随时间延长大鼠HYP有升高的趋势;模型组大鼠血清PⅠCP浓度显著升高,在第4、8和12周时差异有统计学意义(P0.05);模型组大鼠血清PⅢNP浓度显著升高,CTX-Ⅰ浓度显著降低,差异均有统计学意义(P0.01)。结论:压力超负荷状态下,心肌胶原代谢紊乱,且随时间的延长心肌呈不同程度的纤维化表现。
[Abstract]:Objective: to investigate the dynamic changes of myocardial collagen metabolism in pressure overload rats. Methods: the pressure overload rat model was established by abdominal aortic coarctation, and the sham-operated group was used as control group. The rats were measured at the 3rd, 4th, 8th and 12th week after operation to weigh the body mass, heart mass and left ventricular mass. Cardiac mass index (heart mass index,HMI) and left ventricular mass index (left ventricle mass index,LVMI) were calculated. Myocardial collagen deposition was observed by Masson staining and hydroxyproline (hydroxyproline,HYP) content was detected by alkaline hydrolysis. Serum (procollagen type 鈪，

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