芳香化酶（CYP19A1）与绝经后女性冠心病的关联性研究

发布时间：2019-03-02 12:25

【摘要】：目的:芳香化酶基因CYP19A1基因多态性与冠心病的相关性目前仍然不清楚,本研究的目的是探讨CYP19A1基因单核苷酸多态性在汉族、维吾尔族中与冠心病和循环性激素水平及芳香化酶水平之间的关系。方法:本研究纳入1064例汉族(614例男性,450例女性)和790例维吾尔族人(484例男性,306例女性),在从中筛选出498例绝经后女性(265例汉族,233例维吾尔族)。选择了4个多态性位点(rs2236722,rs2304463,rs4646,rs4275794)利用(iMLDR)技术进行基因分型。利用放射免疫法检测雌二醇和睾酮;利用ELISA试剂盒检测人血中芳香化酶水平。结果:在维吾尔族人群中,发现rs2236722和rs4646基因分型和冠心病有显著的关联性(P≤0.03,P≤0.001),其中rs4646的显性模型(CCvsCA+AA)和相加模型(CAvs.CC+AA)具有明显关联(全部P≤0.001)。同时也发现在绝经后女性中rs4646的基因型与性激素和芳香化酶水平有显著关联性(P≤0.001),对循环性激素和方向化酶水平进行逻辑回归调整混杂因素后和冠心病仍然有显著关联(雌二醇:OR=0.777,95%CI:0.717-0.842,P=0.000;芳香化酶水平:OR=0.953,95%CI:0.944-0.963,P=0.000),利用一般线性模型分析rs4646和激素水平的交互分析发现,rs4646与芳香化酶水平显著相关(P=0.026)。结论:芳香化酶基因CYP19A1的rs2236722和rs4646与冠心病、性激素、芳香化酶水平具有显著关联性。
[Abstract]:Objective: the association between CYP19A1 gene polymorphism of aromatase gene and coronary heart disease (CHD) is still unclear. The purpose of this study was to investigate the single nucleotide polymorphism of CYP19A1 gene in Han nationality. Relationship between coronary heart disease, circulating sex hormone levels and aromatase levels in Uighur. Methods: 1064 cases of Han nationality (614 males, 450 females) and 790 Uighurs (484 males and 306 females) were selected from this study. Among them, 498 postmenopausal women (265Han and 233Uyghur) were selected. Four polymorphic loci (rs2236722,rs2304463,rs4646,rs4275794) were selected for genotyping by (iMLDR). Estradiol and testosterone were measured by radioimmunoassay and aromatase levels in human blood were detected by ELISA kit. Results: there was a significant correlation between rs2236722 and rs4646 genotyping and coronary heart disease (CHD) in Uygur population (P < 0.001). There was a significant correlation between the dominant model (CCvsCA AA) of rs4646 and the additive model (CAvs.CC AA) (all P 鈮，

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