MMP-1、TIMP-1及TGF-β1在风湿性心脏病瓣膜组织中的表达及意义

发布时间：2019-03-17 10:30

【摘要】：目的探讨基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶组织抑制剂-1(TIMP-1)及转化生长因子-β1(TGF-β1)在风湿性心脏病(RHD)瓣膜组织中的表达及意义。方法采用Western blot及RT-PCR法检测RHD瓣膜组织(实验组,n=30)及无RHD瓣膜组织(对照组,n=15)中MMP-1、TIMP-1及TGF-β1蛋白与mRNA的表达,碱水解法检测胶原代谢情况,Masson法进行胶原纤维染色并检测胶原容积分数(CVF)。分析MMP-1、TIMP-1及TGF-β1与CVF的相关性。结果 MMP-1及TGF-β1蛋白和mRNA在实验组中的表达高于对照组,而TIMP-1蛋白和mRNA在实验组中的表达低于对照组,差异有统计学意义(P0.05)。实验组胶原含量及CVF均高于对照组(P0.05)。相关性分析显示,MMP-1与TGF-β1呈正相关,TIMP-1与TGF-β1呈负相关,MMP-1及TGF-β1与CVF呈正相关,TIMP-1与CVF呈负相关。结论 MMP-1、TGF-β1和TIMP-1参与了RHD瓣膜病变的纤维化进展。
[Abstract]:Aim to investigate the expression and significance of matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase-1 (TIMP-1) and transforming growth factor-尾 1 (TGF- 尾 1) in (RHD) valve tissue of rheumatic heart disease. Methods the expression of MMP-1,TIMP-1, TGF- 尾 1 protein and mRNA in RHD valvular tissue (experimental group, n = 30) and non-RHD valvular tissue (n = 15) were detected by Western blot and RT-PCR methods, and collagen metabolism was detected by alkaline hydrolysis. Masson staining of collagen fibers and detection of collagen volume fraction (CVF). The correlation between MMP-1,TIMP-1, TGF- 尾 1 and CVF was analyzed. Results the expression of MMP-1, TGF- 尾 1 protein and mRNA in the experimental group was higher than that in the control group, while the expression of TIMP-1 protein and mRNA in the experimental group was lower than that in the control group (P0.05). The collagen content and CVF in the experimental group were higher than those in the control group (P0.05). The correlation analysis showed that MMP-1 was positively correlated with TGF- 尾 1, TIMP-1 was negatively correlated with TGF- 尾 1, MMP-1 and TGF- 尾 1 were positively correlated with CVF, and TIMP-1 was negatively correlated with CVF. Conclusion MMP-1,TGF- 尾 1 and TIMP-1 are involved in the progression of RHD valvular fibrosis.
【作者单位】：福建医科大学附属第一医院心脏外科;福建医科大学附属第一医院超声科;
【基金】：国家自然科学基金(No.81171360)资助
【分类号】：R541.2

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