5-羟色胺对大鼠肺动脉外膜成纤维细胞增殖、迁移及α-平滑肌肌动蛋白表达的影响

发布时间：2019-03-20 17:38

【摘要】：目的探讨5-羟色胺(5-HT)对大鼠肺动脉外膜成纤维细胞增殖、迁移和α-平滑肌肌动蛋白(α-SMA)表达的影响。方法分离并培养大鼠肺动脉外膜成纤维细胞。将体外培养的大鼠肺动脉外膜成纤维细胞分为1~6组,2~6组分别加入10~(-9)、10~(-8)、10~(-7)、10~(-6)、10~(-5) mol/L 5-HT,1组加等量DMEM培养基,继续培养24 h。采用MTT法观察各组细胞增殖情况(OD490值),采用Transwell小室实验观察各组细胞迁移能力(穿膜细胞数),采用Western blotting法检测各组细胞α-SMA表达。结果 1~6组细胞OD490值分别为0.72±0.04、0.79±0.04、0.84±0.05、0.87±0.04、0.93±0.03、0.94±0.03,穿膜细胞数分别为(9.2±1.6)、(17.2±1.7)、(26.0±2.9)、(34.4±2.5)、(63.6±6.0)、(50.4±6.0)个。OD490值、穿膜细胞数:5组4组3组2组1组,P均0.05;5、6组OD490值、穿膜细胞数比较,P均0.05。1~6组细胞α-SMA相对表达量分别为1.01±0.21、1.18±0.35、1.91±0.28、2.37±0.42、2.78±0.33、2.13±0.40。细胞α-SMA相对表达量:5组4组3组2组1组,P均0.05;6组低于5组,P0.05。结论 10-9~10-6mol/L 5-HT可呈剂量依赖性促进大鼠肺动脉外膜成纤维细胞增殖、迁移和α-SMA表达。
[Abstract]:Aim to investigate the effects of 5-hydroxytryptamine (5-HT) on proliferation, migration and 伪-smooth muscle actin (伪-SMA) expression of rat pulmonary artery adventitia fibroblasts. Methods Rat pulmonary adventitial fibroblasts were isolated and cultured. The cultured rat pulmonary artery adventitial fibroblasts were divided into 1 and 6 groups. Group 2 was supplemented with 10 ~ (- 9), 10 ~ (- 8), 10 ~ (- 7), 10 ~ (- 6) and 10 ~ (- 5) mol/L 5 mol/L respectively. The cells were cultured for 24 hours. Cell proliferation (OD490 value) was observed by MTT method, migration capacity (number of transmembrane cells) was observed by Transwell chamber test, and 伪-SMA expression was detected by Western blotting method. Results the OD 490 values were 0.72 卤0.04, 0.79 卤0.04, 0.84 卤0.05, 0.87 卤0.04, 0.93 卤0.03, 0.94 卤0.03 and (9.2 卤1.6), (17.2 卤1.7), (26.0 卤2.9), (34.4 卤2.5), respectively. (63.6 卤6.0), (50.4 卤6.0.OD 490) and number of perforating cells: 5 groups, 4 groups, 3 groups, 1 group, 2 groups, 1 group (P < 0.05); 5. The relative expression of 伪-SMA was 1.01 卤0.21, 1.18 卤0.35,1.91 卤0.28,2.37 卤0.42, 2.78 卤0.33,2.13 卤0.40 in the 6 groups (P = 0.05.1), the OD 490 value and the number of perforating cells were 1.01 卤0.21, 1.18 卤0.35,1.91 卤0.28,2.37 卤0.42, 2.78 卤0.33,2.13 卤0.40, respectively. The relative expression of 伪-SMA: 5 groups, 4 groups, 3 groups, 2 groups, 1 group, P < 0.05, P < 0.05. Conclusion 10-9~10-6mol/L 5-HT can promote the proliferation, migration and 伪-SMA expression of rat pulmonary adventitial fibroblasts in a dose-dependent manner.
【作者单位】：陕西省人民医院;西安交通大学第一附属医院;
【基金】：国家自然科学基金资助项目(81170294)
【分类号】：R544.1

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