绿茶多酚对人肝癌细胞低密度脂蛋白受体功能的影响及机制

发布时间：2019-04-02 15:55

【摘要】：【目的】探讨绿茶多酚主要活性成分表没食子儿茶素没食子酸酯(EGCG)对人肝癌细胞HepG2低密度脂蛋白受体(LDLR)功能的影响及其机制。【方法】用CCK8试剂盒检测EGCG对HepG2细胞最适浓度及增殖活性的影响;用Western Blotting技术检测HepG2细胞LDLR蛋白及前蛋白转化酶枯草杆菌蛋白酶9(PCSK9)蛋白表达水平;用荧光显微镜观察HpeG2细胞对低密度脂蛋白胆固醇(LDL)的摄取水平。【结果】EGCG可通过上调LDLR蛋白表达(P0.05),及下调PCSK9蛋白表达(P0.05),促进HepG2细胞摄取LDL(P0.05)。【结论】EGCG可上调HepG2细胞LDLR蛋白表达,其可能机制是通过下调HepG2细胞PCSK9蛋白的表达,从而减少其对LDLR蛋白的降解,为冠心病降脂治疗提供新的途径。
[Abstract]:[objective] to investigate the effect of epigallocatechin gallate (EGCG), a major active component of green tea polyphenols, on the (LDLR) function of low density lipoprotein receptor (LDL) receptor in human hepatocellular carcinoma cell line HepG2 and its mechanism. [methods] CCK8 kit was used to detect the effect of epigallocatechin gallate. The effect of EGCG on the optimal concentration and proliferative activity of HepG2 cells; The expression levels of LDLR protein and PCSK9 protein in HepG2 cells were detected by Western Blotting technique. The uptake of low density lipoprotein cholesterol (LDL) in HpeG2 cells was observed by fluorescence microscope. [results] EGCG could up-regulate the expression of LDLR protein (P0.05) and down-regulate the expression of PCSK9 protein (P0.05). [conclusion] EGCG can up-regulate the expression of LDLR protein in HepG2 cells by down-regulating the expression of PCSK9 protein in HepG2 cells, thereby reducing the degradation of LDLR protein. [conclusion] EGCG can up-regulate the expression of LDLR protein in HepG2 cells (P0.05). It provides a new way for the treatment of coronary heart disease.
【作者单位】：中山大学附属第一医院心内科;
【基金】：广东省财厅科技项目(2014SC106)
【分类号】：R541.4

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