动脉粥样硬化小鼠主动脉平滑肌上瞬时受体电位通道-1-大电导钙离子激活钾通道信号复合体分子表达量的变化研究

发布时间：2019-06-13 19:49

【摘要】：目的:观察动脉粥样硬化(AS)小鼠主动脉平滑肌上瞬时受体电位通道-1-大电导钙离子激活钾通道(TRPC1-BK)信号复合体分子表达量的变化。方法:AS组采用载脂蛋白E基因缺陷(ApoE~(-/-))小鼠,对照组采用野生型C57BL/6J小鼠,每组10只,分别采用高脂饲料和普通饲料喂养10周,处死后分离主动脉血管平滑肌组织,提取总核糖核酸(RNA)及总蛋白。使用反转录-聚合酶链式反应(RT-PCR)、蛋白免疫印迹(Western blot)及免疫组化染色等方法检测并比较两组TRPC1、大电导钙离子激活钾通道α亚单位(BKα)及大电导钙离子激活钾通道β_1亚单位(BKβ_1)亚基信使核糖核酸(mRNA)和蛋白表达量的差异。结果:AS组AS模型建立成功。RT-PCR检测结果:与对照组比,AS组TRPC1的mRNA表达量增多(P0.05),BKα和BKβ_1亚基的mRNA表达量减少(P均0.01)。Western blot法检测结果:与对照组比,AS组TRPC1蛋白表达量增多(P0.05),BKα和BKβ_1蛋白表达量减少(P均0.01)。免疫组化染色检测结果:TRPC1蛋白表达量增多(P0.01),BKα蛋白表达量减少(P0.01),BKβ_1蛋白表达量减少(P0.05)。结论:AS组小鼠主动脉平滑肌组织中TRPC1-BK信号复合体的mRNA和蛋白表达量均受影响。推测TRPC1-BK信号复合体有可能成为治疗AS相关性血管疾病的一个新靶点。
[Abstract]:Aim: to observe the expression of transient receptor potential channel-1-large conduction calcium activated potassium channel (TRPC1-BK) signal complex in aortic smooth muscle of atherosclerotic (AS) mice. Methods: apolipoprotein E gene deficient (ApoE~ (- / -) mice were used in AS group and wild type C57BL/6J mice in control group, 10 mice in each group were fed with high fat diet and normal diet for 10 weeks, respectively. Aortic vascular smooth muscle tissue was isolated and total ribonucleic acid (RNA) and total protein were extracted after death. Reverse transcription polymerase chain reaction (RT-PCR), Western imprinting (Western blot) and immunohistochemical staining were used to detect and compare the expression of large conductivity calcium channel 伪 subunit (BK 伪) and large conductivity calcium channel 尾 1 subunit (BK 尾 1) subunit messenger RNA (mRNA) and protein between the two groups. Results: the AS model of AS group was successfully established. RT-PCR results: compared with the control group, the mRNA expression of TRPC1 in AS group increased (P 0.05), while the mRNA expression of BK 伪 and BK 尾 _ 1 subunits decreased (P < 0.01). Compared with the control group, the expression of TRPC1 protein increased (P 0.05), and the expression of BK 伪 and BK 尾 _ 1 protein decreased in AS group. The results of Immunohistochemical staining showed that the expression of TRPC1 protein increased (P 0.01), the expression of), BK 伪 protein decreased (P 0.01), and the expression of), BK 尾 _ 1 protein decreased (P 0.05). Conclusion: the mRNA and protein expression of TRPC1-BK signal complex in aortic smooth muscle of mice in AS group are affected. It is speculated that TRPC1-BK signal complex may be a new target for the treatment of AS related vascular diseases.
【作者单位】：安徽医科大学中国人民解放军第一零五医院心内二科;
【分类号】：R543.5

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