Notch1和TACE在腹主动脉瘤血浆中的变化及意义
发布时间：2019-06-24 13:22
【摘要】：研究背景及目的：腹主动脉瘤是多因素相互作用的结果,其显著病理学特点是炎症和动脉粥样硬化。研究发现一些生物学标记物与腹主动脉瘤相关,但是哪一个标记物是诊断腹主动脉瘤的最佳指标,目前没有共识。动物实验研究已经证实Notch信号在腹主动脉瘤发病机制中起重要作用。腹主动脉瘤患者血中的Notch配体,Notch受体和α肿瘤坏死因子转移酶(TACE)还没有被检测过。本研究首次检测腹主动脉瘤患者血浆Jagged1, Notch受体和TACE,研究其与腹主动脉瘤的关系,是否是诊断腹主动脉瘤的可靠生物学标记物。实验方法：入组42例腹主动脉瘤患者和36例非腹主动脉瘤患者。对两组的主动脉直径和临床危险因素进行比较。酶联免疫吸附试验(ELISA)检测两组患者血浆Jagged1, Notch受体(Notch 1-4)和TACE浓度。通过Logistic回归和受试者操作曲线(ROC)分析血浆Notch1和TACE在诊断AAA中的价值。免疫荧光共定位Notch 1和TACE在腹主动脉瘤组织的表达。结果：腹主动脉瘤组血浆Notch1和TACE浓度明显高于对照组(Notch 1,177.3±19.84 pg/ml vs.109.8 ± 5.68 pg/ml, P=0.001; TACE,2395±259.0 pg/ml vs.1113±215.2 pg/ml, P0.0001) 。通过分析ROC曲线下而积(area under the curve, AUC),血浆Notch1诊断腹主动脉瘤的AUC是0.878(95% CI0.771 to 0.985,P0.0001)TACE诊断腹主动脉瘤的AUC是0.804(95% CI0.669 to 0.94,P=0.001)。联合检测Notch1和TACE能够提高诊断腹主动脉瘤的准确性(AUC 0.984,95%CI 0.955 to1.013,P0.0001)。二者联合检测的预测可能性界值0.7的敏感性是90.5%,特异性达到100%。免疫荧光共定位发现Notch1和TACE在腹主动脉瘤组织高表达,且主要分布在CD68+巨噬细胞。结论：本研究首次发现Notch1和TACE腹主动脉瘤血浆中高表达。Notch1和TACE是诊断腹主动脉瘤的可靠生物学标记物。联合检测Notch1和TACE能够提高诊断的敏感性和特异性。同时也证实了Notch信号在腹主动脉瘤发病中的作用,该通路可以作为治疗的靶点。
[Abstract]:Background and objective: abdominal aortic aneurysm is the result of multifactor interaction, and its significant pathological features are inflammation and atherosclerosis. Some biological markers have been found to be associated with abdominal aortic aneurysm, but there is no consensus as to which marker is the best index for the diagnosis of abdominal aortic aneurysm. Animal experimental studies have confirmed that Notch signal plays an important role in the pathogenesis of abdominal aortic aneurysm. Notch ligands, Notch receptors and 伪 tumor necrosis factor transferase (TACE) have not been detected in the blood of patients with abdominal aortic aneurysm. In this study, the relationship between plasma Jagged1, Notch receptor and TACE, in patients with abdominal aortic aneurysm and abdominal aortic aneurysm was detected for the first time, and whether it was a reliable biological marker for the diagnosis of abdominal aortic aneurysm. Methods: 42 patients with abdominal aortic aneurysm and 36 patients with non-abdominal aortic aneurysm were enrolled in the study. The aortic diameter and clinical risk factors were compared between the two groups. Plasma Jagged1, Notch receptor (Notch 1 鈮，
本文编号：2505089
[Abstract]:Background and objective: abdominal aortic aneurysm is the result of multifactor interaction, and its significant pathological features are inflammation and atherosclerosis. Some biological markers have been found to be associated with abdominal aortic aneurysm, but there is no consensus as to which marker is the best index for the diagnosis of abdominal aortic aneurysm. Animal experimental studies have confirmed that Notch signal plays an important role in the pathogenesis of abdominal aortic aneurysm. Notch ligands, Notch receptors and 伪 tumor necrosis factor transferase (TACE) have not been detected in the blood of patients with abdominal aortic aneurysm. In this study, the relationship between plasma Jagged1, Notch receptor and TACE, in patients with abdominal aortic aneurysm and abdominal aortic aneurysm was detected for the first time, and whether it was a reliable biological marker for the diagnosis of abdominal aortic aneurysm. Methods: 42 patients with abdominal aortic aneurysm and 36 patients with non-abdominal aortic aneurysm were enrolled in the study. The aortic diameter and clinical risk factors were compared between the two groups. Plasma Jagged1, Notch receptor (Notch 1 鈮，
本文编号：2505089
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