阿托伐他汀诱导血红素氧合酶1表达对血管内皮细胞抗炎症损伤能力的影响

发布时间：2019-07-06 14:08

【摘要】：目的探讨阿托伐他汀能否通过诱导血红素氧合酶1(HO-1)表达增强血管内皮细胞抗炎症损伤的能力。方法体外培养人脐静脉内皮细胞(HUVEC),随机分为5组,分别予以不同浓度的阿托伐他汀(0、1、2、5、10μmol/L)孵育24 h,检测HO-1蛋白表达量和上清液胆红素浓度。另将HUVEC随机分5组,对照组(等量纯培养基)、大剂量他汀组(阿托伐他汀10μmol/L)、小剂量他汀组(阿托伐他汀2μmol/L)、HO-1阻断组(阿托伐他汀10μmol/L+Zn PP IX 10μmol/L)、胆红素组(胆红素5μmol/L)孵育细胞24 h后,各组均加入终浓度为10 ng/mL的TNF-α孵育细胞12 h。检测细胞上清液乳酸脱氢酶(LDH)、单核细胞趋化蛋白1(MCP-1)、丙二醛(MDA)、一氧化氮(NO)浓度并检测细胞活力和凋亡率。结果与0μmol/L浓度组比较,阿托伐他汀2、5、10μmol/L浓度组HO-1蛋白的表达及胆红素水平增高(P0.05或0.01)。与对照组比较,加用阿托伐他汀或胆红素预处理组上清液MCP-1、MDA、LDH水平降低且NO水平升高,细胞活力增强,细胞凋亡率降低(P0.05或0.01),HO-1阻断组上述各项指标均无明显变化(P均0.05)。结论阿托伐他汀具有诱导HUVEC血红素氧合酶1(HO-1)表达增强血管内皮细胞抗炎症损伤的能力,且此作用可能存在剂量依赖性。
[Abstract]:Objective to investigate whether Atto statins can enhance the ability of anti-inflammatory injury of vascular endothelial cells by inducing the expression of heme oxygenase-1 (HO-1). Methods Human umbilical vein endothelial cells (HUVEC),) cultured in vitro were randomly divided into 5 groups and incubated with different concentrations of Atto statins (0, 1, 2, 5, 10 渭 mol / L) for 24 h. The expression of HO-1 protein and the concentration of bilirubin in the culture medium were detected. In addition, HUVEC was randomly divided into 5 groups, control group (equal amount of pure culture medium), high dose statins group (Atto statins 10 渭 mol / L), low dose statins group (Atto varastatin 2 渭 mol / L), HO-1 blocking group (atorvastatin 10 渭 mol / L Zn PP IX 10 渭 mol / L), cholin group) 24 h, and the cells were incubated with TNF- 伪 at the final concentration of 10 ng/mL for 12 h. The concentrations of lactic dehydrogenase (LDH), monocyte chemoattractant protein 1 (MCP-1) and malondialdehyde (MDA), nitric oxide (NO) in cell culture were measured, and the cell activity and apoptosis rate were detected. Results compared with 0 渭 mol / L group, the expression of HO-1 protein and the level of bilirubin in 5 and 10 渭 mol / L groups were higher than those in 0 渭 mol / L group (P 0.05 or 0.01). Compared with the control group, MCP-1,MDA,LDH level decreased, NO level increased, cell activity increased and apoptosis rate decreased in the treatment group supplemented with Atto Vastatin or bilirubin pretreatment group, but there was no significant change in the above indexes in the HO-1 blocking group (all P 0.05). Conclusion Atto statins can induce the expression of HUVEC heme oxygenase 1 (HO-1) to enhance the anti-inflammatory injury of vascular endothelial cells, and this effect may be dose-dependent.
【作者单位】：西南医科大学附属医院;
【分类号】：R54

【相似文献】