动脉粥样硬化患者血清miR-520b表达水平及相关机制
发布时间：2019-07-10 15:52
【摘要】：目的探讨动脉粥样硬化患者血清miR-520b表达水平及相关作用机制。方法选取该院收治的动脉粥样硬化患者42例,收集同期42名健康老年人为对照。qRT-PCR检测外周血清miR-520b表达水平。经targetscan和miRanda数据库预测importin 8(IPO8)为miR-520b的可能靶基因,通过双荧光素酶报告基因检测证实;体外细胞实验采用Western印迹检测miR-520b对人血管内皮细胞IPO8表达的影响;CCK-8法和Tranwells实验检测miR-520b对人血管平滑肌细胞增殖、迁移能力的影响。结果动脉粥样硬化患者血清miR-520b相对表达量低于健康老年人(P0.05)。双荧光素酶报告基因检测证实IPO8是miR-520b的直接作用靶基因;转染miR-520b类似物人血管内皮细胞IPO8蛋白相对表达量低于类似物阴性对照组(P0.05);转染特异性miR-520b抑制物后,血管内皮细胞中IPO8蛋白相对表达量高于抑制物对照组(P0.05)。提升miR-520b表达后人血管平滑肌细胞增殖和迁徙能力均低于相应阴性对照(P0.05);抑制miR-520b表达后人血管平滑肌细胞增殖和迁徙能力均高于相应阴性对照,差异有统计学意义(P0.05)。结论动脉粥样硬化患者血清miR-520b表达水平明显降低;miR-520b可通过阻断NF-κB信号通路及抑制血管平滑肌细胞增殖、迁徙来发挥抗动脉粥样硬化的作用。
文内图片：
图片说明：miＲ-520b对人血管内皮细胞中IPO8蛋白表达的影响
[Abstract]:Objective to investigate the expression of serum miR-520b in patients with atherosclerosis and its related mechanism. Methods 42 patients with atherosclerosis were selected and 42 healthy elderly were collected as control. QRT-PCR was used to detect the expression of miR-520b in peripheral serum. Importin 8 (IPO8) was predicted by targetscan and miRanda database as the possible target gene of miR-520b, confirmed by double luciferase reporter gene detection, the effect of miR-520b on the expression of IPO8 in human vascular endothelial cells was detected by Western imprinting in vitro, and the effect of miR-520b on the proliferation and migration of human vascular smooth muscle cells was detected by CCK- 8 assay and Tranwells assay. Results the relative expression of serum miR-520b in patients with atherosclerosis was lower than that in healthy elderly (P 0.05). The detection of double luciferase reporter gene confirmed that IPO8 was the direct target gene of miR-520b; the relative expression of IPO8 protein in human vascular endothelial cells was lower than that in the control group (P 0.05), and the relative expression of IPO8 protein in vascular endothelial cells was higher than that in the inhibitor control group (P 0.05). The proliferation and migration ability of human vascular smooth muscle cells after increasing the expression of miR-520b was lower than that of the corresponding negative control (P 0.05), and the proliferation and migration ability of human vascular smooth muscle cells after inhibiting the expression of miR-520b was higher than that of the corresponding negative control, the difference was statistically significant (P 0.05). Conclusion the expression of miR-520b in serum of patients with atherosclerosis is significantly decreased, and miR-520b can play an anti-atherogenic role by blocking NF- kappa B signal pathway and inhibiting the proliferation and migration of vascular smooth muscle cells.
【作者单位】： 承德市中心医院心血管内科;
【基金】：河北省科学技术厅项目(20143217)
【分类号】：R543.5
本文编号：2512705
文内图片：
图片说明：miＲ-520b对人血管内皮细胞中IPO8蛋白表达的影响
[Abstract]:Objective to investigate the expression of serum miR-520b in patients with atherosclerosis and its related mechanism. Methods 42 patients with atherosclerosis were selected and 42 healthy elderly were collected as control. QRT-PCR was used to detect the expression of miR-520b in peripheral serum. Importin 8 (IPO8) was predicted by targetscan and miRanda database as the possible target gene of miR-520b, confirmed by double luciferase reporter gene detection, the effect of miR-520b on the expression of IPO8 in human vascular endothelial cells was detected by Western imprinting in vitro, and the effect of miR-520b on the proliferation and migration of human vascular smooth muscle cells was detected by CCK- 8 assay and Tranwells assay. Results the relative expression of serum miR-520b in patients with atherosclerosis was lower than that in healthy elderly (P 0.05). The detection of double luciferase reporter gene confirmed that IPO8 was the direct target gene of miR-520b; the relative expression of IPO8 protein in human vascular endothelial cells was lower than that in the control group (P 0.05), and the relative expression of IPO8 protein in vascular endothelial cells was higher than that in the inhibitor control group (P 0.05). The proliferation and migration ability of human vascular smooth muscle cells after increasing the expression of miR-520b was lower than that of the corresponding negative control (P 0.05), and the proliferation and migration ability of human vascular smooth muscle cells after inhibiting the expression of miR-520b was higher than that of the corresponding negative control, the difference was statistically significant (P 0.05). Conclusion the expression of miR-520b in serum of patients with atherosclerosis is significantly decreased, and miR-520b can play an anti-atherogenic role by blocking NF- kappa B signal pathway and inhibiting the proliferation and migration of vascular smooth muscle cells.
【作者单位】： 承德市中心医院心血管内科;
【基金】：河北省科学技术厅项目(20143217)
【分类号】：R543.5
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1 王凯;肝X受体α在同型半胱氨酸促进动脉粥样硬化中的作用机制研究[D];宁夏医科大学;2017年
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