心肌梗死与心室重构中miRNA和转录因子共调控网络分析

发布时间：2024-03-07 19:41

　　心肌梗死是当今社会中引发死亡的重要因素，因此开发新的治疗方法是临床和研究心肌梗死的重要目标。心肌梗死涉及到的相关基因和过程众多，其中的调控关系也很复杂。本文中，我们基于基因调控网络的方法对心肌梗死中的基因调控模式进行了综合分析。首先，我们从多个资源如：PubMed、CADgene、GWAS、HMDD and miR2disease收集了与心肌梗死相关的基因和miRNA。其次，我们预测了人类所有转录因子、心肌梗死相关的miRNA及基因间的调控关系。转录因子对心肌梗死相关的miRNA和基因的调控，我们从UCSC（hg18）获得了预测的转录因子结合位点以及从TRANSFAC和ChIP-seq数据获得了转录因子验证的靶基因。通过整合两个miRNA靶基因预测软件TargetScan和miRanda的结果，得到了miRNA预测的靶基因，并整合miR2Disease、miRTarBase、miRecords和TarBase四个数据库收集的实验验证的miRNA靶基因，我们获得了心肌梗死相关miRNA的靶基因。最后，我们使用上述调控关系构建了心肌梗死特异的miRNA-转录因子共调控网络。该网络共包含了2...

【文章页数】：68 页

【学位级别】：硕士

【文章目录】：
Abstract
摘要
Contents
CHAPTER 1: INTRODUCTION AND LITERATURE REVIEW
    1.1 INTRODUCTION
    1.2 MicroRNAs and Transcription factors in cardiovascular diseases
    1.3 Myocardial Infarction
    1.4 Ventricular Remodeling
    1.5 Current cardioprotective agents and therapeutic strategies essential in myocardial ischemia/reperfusion (I/R) injury
    1.6 Construction method of microRNA and Transcription factor co‐regulatory networks
    1.7 Study Objectives
CHAPTER 2: METHODOLOGY
    2.1 MI candidate genes and miRNAs
    2.2 Identification of miRNA and TF targets
    2.3 Network and hub analysis
    2.4 Functional Annotation of miRNA targets and pathway analysis
CHAPTER 3: RESULTS
    3.1 Basic information of MI related miRNAs and genes curated from literature and databases ..
    3.2 MI miRNA‐TF regulatory network and its characteristics
    3.3 Overlap between hub genes in the MI miRNA‐TF regulatory network
    3.4 Post‐MI subnetwork and overlap between MI hub and Post‐MI hub subnetworks
    3.5 Canonical pathway enrichment of miRNA and TF targets
    3.6 NFAT and Hypertrophy of the heart specific regulatory network in MI
    3.7 Cellular Model of essential components in MI progression
CHAPTER 4: DISCUSSION AND CONCLUSION
Acknowledgements
References



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