Dicer1低表达与鼻咽癌患者预后的关联性研究

发布时间：2018-01-03 03:41

本文关键词：Dicer1低表达与鼻咽癌患者预后的关联性研究　出处：《临床耳鼻咽喉头颈外科杂志》2015年02期 　论文类型：期刊论文

更多相关文章： Dicer 低表达 鼻咽癌 预后 生物标志物

【摘要】：目的:Dicer1在RNA干扰的起始阶段起着重要作用,本研究旨在评估Dicer1在鼻咽癌组织中的表达及预后价值。方法:采用免疫组织化学染色法检测276例鼻咽癌活检标本的Dicer1蛋白表达,利用定量RTPCR法分析56例鼻咽癌活检标本和11例鼻咽炎组织中Dicer1mRNA水平,采用Cox回归分析确定独立预后因素,建立预后评分模型进行生存预测。结果:Dicer1的mRNA和蛋白在鼻咽癌组织中表达水平均明显偏低,Dicer1的mRNA表达水平与蛋白表达水平呈正相关。Dicer1的低表达与鼻咽癌患者远处转移的发生(P0.01)以及患者死亡率(P0.05)显著相关。Dicer1低表达与鼻咽癌患者的总体生存期(HR=2.32,95%CI:1.30~4.14,P0.01)和无远处转移生存率(HR=2.56,95%CI:1.39~4.74,P0.01)亦显著相关。此外,多变量分析显示Dicer1低表达和TNM分期是鼻咽癌患者总体生存期和无远处转移生存率的独立预后因素。Dicer1表达和TNM分期相结合的预后评分模型比Dicer1表达单独模型或TNM分期单独模型拥有更好的预后价值(P0.05)。结论:Dicer1mRNA和蛋白表达水平在鼻咽癌组织中均明显偏低,Dicer1的低表达可能是鼻咽癌患者新的预后标志物。
[Abstract]:Objective: dicer1 plays an important role in the initial phase of RNA interference. The purpose of this study was to evaluate the expression and prognostic value of Dicer1 in nasopharyngeal carcinoma (NPC). Methods: immunohistochemical staining was used to detect the expression of Dicer1 protein in 276 NPC biopsy specimens. The levels of Dicer1mRNA in 56 nasopharyngeal carcinoma biopsy specimens and 11 nasopharyngitis tissues were analyzed by quantitative RTPCR method. Cox regression analysis was used to determine the independent prognostic factors. Results the expression of mRNA and protein in nasopharyngeal carcinoma was significantly lower than that in control group. There was a positive correlation between the expression of mRNA and protein in Dicer1. The low expression of Dicer1 was associated with the distant metastasis of nasopharyngeal carcinoma (P0.01) and the mortality (P < 0.05). P0.05) there was a significant correlation between the low expression of .Dicer1 and the overall survival time of patients with nasopharyngeal carcinoma (HR2.32). 95 CI: 1.30 / 4.14 P0.01) and distant metastasis-free survival rate HR2.566 / 95 CI: 1.39 / 4.74. P0.01) was also significantly correlated. Multivariate analysis showed that low expression of Dicer1 and TNM staging were independent prognostic factors of overall survival and distant metastasis-free survival in patients with nasopharyngeal carcinoma. Dicer1 expression combined with TNM staging. The scoring model has better prognostic value than the single Dicer1 model or the TNM staging model. Conclusion the mRNA and protein expression level of 1: Dicer1 in nasopharyngeal carcinoma is significantly lower than that in nasopharyngeal carcinoma. Low expression of Dicer1 may be a new prognostic marker in patients with nasopharyngeal carcinoma.
【作者单位】：中国医科大学附属第一医院耳鼻咽喉科;
【分类号】：R739.63
【正文快照】： 鼻咽癌(nasopharyngeal carcinoma,NPC)是一种常见的恶性肿瘤,据全球统计数据显示,NPC的发病率存在显著的地域性差异,平均每10万男性的平均年龄标化发病率中,中国南部地区占总发病率的20%~50%,而欧洲地区仅占0.5%。精确的预后能为NPC患者提供更好的治疗选择,但目前被广泛接受

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